1887

Abstract

Previous work has shown that the EzrA protein directly inhibits FtsZ ring assembly, which is required for normal cell division, and that loss of EzrA results in hyperstabilization of the FtsZ polymer . Here, it was found that in -disrupted cells, artificial expression of YneA, which suppresses cell division during the SOS response, and disruption of (), which acts as an effector of nucleoid occlusion, resulted in accumulation of multiple non-constricting FtsZ rings, inhibition of cell division, and synthetic lethality. Overexpression of the essential cell division protein FtsL suppressed the effect of disruption. FtsL overexpression recovered the delayed FtsZ ring constriction seen in -disrupted wild-type cells. Conversely, the absence of EzrA caused lethality in cells producing a lower amount of FtsL than wild-type cells. It has previously been reported that FtsL is recruited to the division site during the later stages of cell division, although its exact role is currently unknown. The results of this study suggest that FtsL and EzrA synergistically regulate the FtsZ ring constriction in . Interestingly, FtsL overexpression also suppressed the cell division inhibition due to YneA expression or Noc inactivation in -disrupted cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.28497-0
2006-04-01
2020-04-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/152/4/1129.html?itemId=/content/journal/micro/10.1099/mic.0.28497-0&mimeType=html&fmt=ahah

References

  1. Anderson D. E, Gueiros-Filho F. J, Erickson H. P. 2004; Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins. J Bacteriol186:5775–5781[CrossRef]
    [Google Scholar]
  2. Chung K. M, Hsu H. H, Govindan S, Chang B. Y. 2004; Transcription regulation of ezrA and its effect on cell division of Bacillus subtilis . J Bacteriol186:5926–5932[CrossRef]
    [Google Scholar]
  3. Daniel R. A, Errington J. 2000; Intrinsic instability of the essential cell division protein FtsL of Bacillus subtilis and a role for DivIB protein in FtsL turnover. Mol Microbiol36:278–289[CrossRef]
    [Google Scholar]
  4. Daniel R. A, Harry E. J, Katis V. L, Wake R. G, Errington J. 1998; Characterization of the essential cell division gene ftsL (yllD) of Bacillus subtilis and its role in the assembly of the division apparatus. Mol Microbiol29:593–604[CrossRef]
    [Google Scholar]
  5. Errington J, Daniel R. A. 2002; Cell division during growth and sporulation. In Bacillus subtilis and its Closest Relatives: from Genes to Cells pp 97–109 Edited by Sonenshein A. L., Hoch J. A., Losick R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Errington J, Daniel R. A, Scheffers D. J. 2003; Cytokinesis in bacteria. Microbiol Mol Biol Rev67:52–65[CrossRef]
    [Google Scholar]
  7. Gueiros-Filho F. J, Losick R. 2002; A widely conserved bacterial cell division protein that promotes assembly of the tubulin-like protein FtsZ. Genes Dev16:2544–2556[CrossRef]
    [Google Scholar]
  8. Haeusser D. P, Schwartz R. L, Smith A. M, Oates M. E, Levin P. A. 2004; EzrA prevents aberrant cell division by modulating assembly of the cytoskeletal protein FtsZ. Mol Microbiol52:801–814[CrossRef]
    [Google Scholar]
  9. Harry E. J. 2001; Bacterial cell division: regulating Z-ring formation. Mol Microbiol40:795–803[CrossRef]
    [Google Scholar]
  10. Hassan A. K. M, Moriya S, Ogura T, Tanaka T, Kawamura F, Ogasawara N. 1997; Suppression of initiation defects of chromosome replication in Bacillus subtilis dnaA and oriC -deleted mutants by integration of a plasmid replicon into the chromosomes. J Bacteriol179:2494–2502
    [Google Scholar]
  11. Hu Z, Lutkenhaus J. 1999; Topological regulation of cell division in Escherichia coli involves rapid pole to pole oscillation of the division inhibitor MinC under the control of MinD and MinE. Mol Microbiol34:82–90[CrossRef]
    [Google Scholar]
  12. Hu Z, Mukherjee A, Pichoff S, Lutkenhaus J. 1999; The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization. Proc Natl Acad Sci U S A96:14819–14824[CrossRef]
    [Google Scholar]
  13. Itaya M. 1992; Construction of a novel tetracycline resistance gene cassette useful as a marker on the Bacillus subtilis chromosome. Biosci Biotechnol56:685–686[CrossRef]
    [Google Scholar]
  14. Kawai Y, Moriya S, Ogasawara N. 2003; Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis . Mol Microbiol47:1113–1122[CrossRef]
    [Google Scholar]
  15. Kim L, Mogk A, Schumann W. 1996; A xylose-inducible Bacillus subtilis integration vector and its application. Gene181:71–76[CrossRef]
    [Google Scholar]
  16. LeDeaux J. R, Grossman A. D. 1995; Isolation and characterization of kinC , a gene that encodes a sensor kinase homologous to the sporulation sensor kinases KinA and KinB in Bacillus subtilis . J Bacteriol177:166–175
    [Google Scholar]
  17. Levin P. A, Kurtser I. G, Grossman A. D. 1999; Identification and characterization of a negative regulator of FtsZ ring formation in Bacillus subtilis . Proc Natl Acad Sci U S A96:9642–9647[CrossRef]
    [Google Scholar]
  18. Levin P. A, Schwartz R. L, Grossman A. D. 2001; Polymer stability plays an important role in the positional regulation of FtsZ. J Bacteriol183:5449–5452[CrossRef]
    [Google Scholar]
  19. Marston A. L, Errington J. 1999; Selection of the midcell division site in Bacillus subtilis through MinD-dependent polar localization and activation of MinC. Mol Microbiol33:84–96[CrossRef]
    [Google Scholar]
  20. Moriya S, Kato K, Yoshikawa H, Ogasawara N. 1990; Isolation of a dnaA mutant of Bacillus subtilis defective in initiation of replication: amount of DnaA protein determines cells' initiation potential. EMBO J9:2905–2910
    [Google Scholar]
  21. Moriya S, Tsujikawa E, Hassan A. K, Asai K, Kodama T, Ogasawara N. 1998; A Bacillus subtilis gene-encoding protein homologous to eukaryotic SMC motor protein is necessary for chromosome partition. Mol Microbiol29:179–187[CrossRef]
    [Google Scholar]
  22. Price K. D, Losick R. 1999; A four-dimensional view of assembly of a morphogenetic protein during sporulation in Bacillus subtilis . J Bacteriol181:781–790
    [Google Scholar]
  23. Raskin D. M, de Boer P. A. J. 1999; MinDE-dependent pole-to-pole oscillation of division inhibitor MinC in Escherichia coli . J Bacteriol181:6419–6424
    [Google Scholar]
  24. Romberg L, Levin P. A. 2003; Assembly dynamics of the bacterial cell division protein FTSZ: poised at the edge of stability. Annu Rev Microbiol57:125–154[CrossRef]
    [Google Scholar]
  25. Sievers J, Errington J. 2000; The Bacillus subtilis cell division protein FtsL localizes to sites of septation and interacts with DivIC. Mol Microbiol36:846–855[CrossRef]
    [Google Scholar]
  26. Vagner V, Dervyn E, Ehrlich S. D. 1998; A vector for systematic gene inactivation in Bacillus subtilis . Microbiology144:3097–3104[CrossRef]
    [Google Scholar]
  27. Wu L. J, Errington J. 2004; Coordination of cell division and chromosome segregation by a nucleoid occlusion protein in Bacillus subtilis . Cell117:915–925[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.28497-0
Loading
/content/journal/micro/10.1099/mic.0.28497-0
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error