1887

Abstract

Regulation of methionine biosynthesis in involves a complex of the MetJ aporepressor protein and -adenosylmethionine (SAM) repressing expression of most genes in the regulon. To test the role of SAM in the regulation of genes directly, SAM pools were depleted by the expression of the cloned plasmid vector-based coliphage T3 SAM hydrolase (SAMase) gene. Cultures with SAMase activity were assayed for expression of the , , , , , , , and genes in cells grown in methionine-rich complete media as well as in defined media with and without -methionine. SAMase activity dramatically induced expression between 11- and nearly 1000-fold depending on the gene assayed for all but and , and these genes were induced over twofold.  : : Tn (aporepressor defective) and  : : Tn (SAM synthetase impaired; produces <5 % of wild-type SAM) strains containing SAMase activity produced even higher gene activity than that seen in comparably prepared cells with wild-type genes for all but in a MetJ-deficient background. The SAMase-mediated hyperinduction of in wild-type cells and of the genes assayed in  : : Tn and  : : Tn cells provokes questions about how other elements such as the MetR activator protein or factors beyond the regulon itself might be involved in the regulation of genes responsible for methionine biosynthesis.

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2006-05-01
2019-11-19
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