1887

Abstract

Identification of serotypes is based on flagellar and somatic antigens. The absence of flagella may consequently affect complete identification of the serotype; here it is shown that serovar Typhimurium exhibits morphological differences dependent on the peptone constituents of the culture medium. Aflagellate salmonella were produced in certain media where the nutritional ingredient was casein-based peptone or gelatin-based peptone; in gelatin-based peptone, aggregates of salmonella were observed. However, in media containing soy-based peptone as the primary nutrient, salmonella displayed a normal flagellated morphology. Transfer of aflagellate salmonella from nutritionally poor media, with casein- or gelatin-based peptone, into rich nutrient broth allowed flagella synthesis, indicating that the aflagellate form is still able to produce flagella. Amino acid sequencing of the peptones producing aflagellate organisms showed a relatively low tyrosine concentration: only 0·03±0·01 g l for gelatin-based buffered peptone water, compared to 0·21±0·01 for soy-based buffered peptone water. Tyrosine is essential for flagellin, which is the subunit of the salmonella flagellar filament. The addition of 200 μM tyrosine to casein-based peptone media produced flagellate salmonella; 2 mM glucose was needed in addition to tyrosine to achieve a similar morphology in gelatin-based media. Therefore, culture media containing less than 1·20 g tyrosine l, and of limited carbohydrate source, when used for serological testing of clinical isolates, may result in an incomplete serological identification.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.28442-0
2006-01-01
2020-03-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/152/1/23.html?itemId=/content/journal/micro/10.1099/mic.0.28442-0&mimeType=html&fmt=ahah

References

  1. Ames G. F. 1964; Uptake of amino acids by Salmonella typhimurium . Arch Biochem Biophys104:1–18[CrossRef]
    [Google Scholar]
  2. Andrews W. H. 1986; Resuscitation of injured Salmonella spp. and coliforms from foods. J Food Prot49:62–75
    [Google Scholar]
  3. Antonucci T. K, Oxender D. L. 1986; The molecular biology of amino-acid transport in bacteria. Adv Microb Physiol28:145–180
    [Google Scholar]
  4. Baylis C. L, MacPhee S, Betts R. P. 2000; Comparison of two commercial preparations of buffered peptone water for the recovery and growth of Salmonella bacteria from foods. J Appl Microbiol89:501–510[CrossRef]
    [Google Scholar]
  5. Bridson E. Y. editor 1995; The Oxoid Manual, 7th edn. Basingstoke: Unipath Ltd;
    [Google Scholar]
  6. Dubois M, Gilles K. A, Hamilton J. K, Rebers P. A, Smith F. 1956; Colorimetric method for determination of sugars and related substances. Anal Chem28:350–356[CrossRef]
    [Google Scholar]
  7. Guard-Petter J. 1997; Induction of flagellation and a novel agar-penetrating flagellar structure in Salmonella enterica grown on solid media: possible consequences for serological identification. FEMS Microbiol Lett149:173–180[CrossRef]
    [Google Scholar]
  8. Harshey R. M, Matsuyama T. 1994; Dimorphic transition in Escherichia coli and Salmonella typhimurium: surface-induced differentiation into hyperflagellate swarmer cells. Proc Natl Acad Sci U S A91:8631–8635[CrossRef]
    [Google Scholar]
  9. Kerridge D. 1959; Synthesis of flagella by amino acid-requiring mutants of Salmonella typhimurium . J Gen Microbiol21:168–179[CrossRef]
    [Google Scholar]
  10. Leifson E, Carhart S. R, Fulton M. 1955; Morphological characteristics of flagella of Proteus and related bacteria. J Bacteriol69:73–82
    [Google Scholar]
  11. Neidhardt F. C, Bloch P. L, Smith D. F. 1974; Culture medium for enterobacteria. J Bacteriol119:736–747
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.28442-0
Loading
/content/journal/micro/10.1099/mic.0.28442-0
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error