1887

Abstract

The structural proteome of KMV, a lytic bacteriophage infecting , was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in KMV and suggest subtle structural differences from phage T7.

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2006-02-01
2019-10-14
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References

  1. Beveridge, T. J. ( 1999; ). Structures of Gram-negative cell walls and their derived membrane vesicles. J Bacteriol 181, 4725–4733.
    [Google Scholar]
  2. Condron, B. G., Atkins, J. F. & Gesteland, R. F. ( 1991; ). Frameshifting in gene 10 of bacteriophage T7. J Bacteriol 173, 6998–7003.
    [Google Scholar]
  3. Dobbins, A. T., George, M., Jr, Basham, D. A., Ford, M. E., Houtz, J. M., Pedulla, M. L., Lawrence, J. G., Hatfull, G. F. & Hendrix, R. W. ( 2004; ). Complete genomic sequence of the virulent Salmonella bacteriophage SP6. J Bacteriol 186, 1933–1944.[CrossRef]
    [Google Scholar]
  4. Goodacre, R., Timmins, E. M., Burton, R., Kaderbhai, N., Woodward, A. M., Kell, D. B. & Rooney, P. J. ( 1998; ). Rapid identification of urinary tract infection bacteria using hyperspectral whole-organism fingerprinting and artificial neural networks. Microbiology 144, 1157–1170.[CrossRef]
    [Google Scholar]
  5. Kovalyova, I. V. & Kropinski, A. M. ( 2003; ). The complete genomic sequence of lytic bacteriophage gh-1 infecting Pseudomonas putida – evidence for close relationship to the T7 group. Virology 311, 305–315.[CrossRef]
    [Google Scholar]
  6. Lavigne, R., Burkal'tseva, M. V., Robben, J. & 7 other authors ( 2003; ). The genome of bacteriophage ϕKMV, a T7-like virus infecting Pseudomonas aeruginosa. Virology 312, 49–59.[CrossRef]
    [Google Scholar]
  7. Lavigne, R., Briers, Y., Hertveldt, K., Robben, J. & Volckaert, G. ( 2004; ). Identification and characterization of a structure-related, thermostable lysozyme from bacteriophage ϕKMV. Cell Mol Life Sci 61, 2753–2759.[CrossRef]
    [Google Scholar]
  8. Lavigne, R., Roucourt, B., Hertveldt, K. & Volckaert, G. ( 2005; ). Characterization of the bacteriophage ϕKMV DNA ligase. Protein Pept Lett 12, 645–648.[CrossRef]
    [Google Scholar]
  9. Mayrand, D. & Grenier, D. ( 1989; ). Biological activities of outer membrane vesicles. Can J Microbiol 35, 607–613.[CrossRef]
    [Google Scholar]
  10. Moak, M. & Molineux, I. J. ( 2000; ). Role of the Gp16 lytic transglycosylase motif in bacteriophage T7 virions at the initiation of infection. Mol Microbiol 37, 345–355.[CrossRef]
    [Google Scholar]
  11. Moak, M. & Molineux, I. J. ( 2004; ). Peptidoglycan hydrolytic activities associated with bacteriophage virions. Mol Microbiol 51, 1169–1183.[CrossRef]
    [Google Scholar]
  12. Molineux, I. J. ( 2001; ). No syringes please, ejection of phage T7 DNA from the virion is enzyme driven. Mol Microbiol 40, 1–8.[CrossRef]
    [Google Scholar]
  13. Roberts, M. D., Martin, N. L. & Kropinski, A. M. ( 2004; ). The genome and proteome of coliphage T1. Virology 318, 245–266.[CrossRef]
    [Google Scholar]
  14. Rohwer, F. & Edwards, R. ( 2002; ). The phage proteomic tree: a genome-based taxonomy for phage. J Bacteriol 184, 4529–4535.[CrossRef]
    [Google Scholar]
  15. Sambrook, J. & Russell, D. W. ( 2001; ). Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  16. Scholl, D., Rogers, S., Adhya, S. & Merril, C. R. ( 2001; ). Bacteriophage K1-5 encodes two different tail fiber proteins, allowing it to infect and replicate on both K1 and K5 strains of Escherichia coli. J Virol 75, 2509–2515.[CrossRef]
    [Google Scholar]
  17. Scholl, D., Kieleczawa, J., Kemp, P., Rush, J., Richardson, C. C., Merril, C., Adhya, S. & Molineux, I. J. ( 2004; ). Genomic analysis of bacteriophages SP6 and K1-5, an estranged subgroup of the T7 Supergroup. J Mol Biol 335, 1151–1171.[CrossRef]
    [Google Scholar]
  18. Shevchenko, A., Wilm, M., Vorm, O., Jensen, O. N., Podtelejnikov, A. V., Neubauer, G., Shevchenko, A., Mortensen, P. & Mann, M. ( 1996a; ). A strategy for identifying gel-separated proteins in sequence databases by MS alone. Biochem Soc Trans 24, 893–896.
    [Google Scholar]
  19. Shevchenko, A., Wilm, M., Vorm, O. & Mann, M. ( 1996b; ). Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal Chem 86, 850–858.
    [Google Scholar]
  20. Spahr, C. S., Davis, M. T., McGinley, M. D. & 10 other authors ( 2001; ). Towards defining the urinary proteome using liquid chromatography-tandem mass spectrometry. I. Profiling an unfractionated tryptic digest. Proteomics 1, 93–107.[CrossRef]
    [Google Scholar]
  21. Taylor, J. A. & Johnson, R. S. ( 1997; ). Sequence database searches via de novo peptide sequencing by tandem mass spectrometry. Rapid Commun Mass Spectrom 11, 1067–1075.[CrossRef]
    [Google Scholar]
  22. Zhang, X., Huang, C., Tang, X., Zhuang, Y. & Hew, C. H. ( 2004; ). Identification of structural proteins from shrimp white spot syndrome virus (WSSV) by 2DE-MS. Protein Struct Funct Bioinformatics 55, 229–235.[CrossRef]
    [Google Scholar]
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