1887

Abstract

is a Gram-negative enterobacterium that has become an important opportunistic pathogen, largely due to its high degree of natural antibiotic resistance. One factor contributing to this natural antibiotic resistance is reduced outer membrane permeability, which is controlled in part by OmpC and OmpF porin proteins. OmpF expression is regulated by , an RNA transcript encoded upstream of the gene, which hybridizes with the transcript to inhibit its translation. Regulation of porin gene expression, as well as that of , was investigated using -galactosidase reporter gene fusions in response to 5, 8 and 10 % sucrose, 1, 5 and 8 mM salicylate, and different pH and temperature values. -Galactosidase activity assays revealed that a lower growth temperature (28 °C), a more basic pH (pH 8), and an absence of sucrose and salicylate induce the transcription of the gene, whereas the induction of is stimulated at a higher growth temperature (42 °C), acidic pH (pH 6), and maximum concentrations of sucrose (10 %) and salicylate (8 mM). In addition, when multiple conditions were tested, temperature had the predominant effect, followed by pH. In this study, it was found that the MicF regulatory mechanism does not play a role in the osmoregulation of the and genes, whereas MicF does repress OmpF expression in the presence of salicylate and high growth temperature, and under low pH conditions.

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2006-02-01
2019-10-20
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