1887

Abstract

The biosynthetic gene cluster of goadsporin, a polypeptide antibiotic containing thiazole and oxazole rings, was cloned from sp. TP-A0584. The cluster contains a structural gene, , and nine (asporin) genes involved in post-translational modification, immunity and transcriptional regulation. Although the gene organization is similar to typical bacteriocin biosynthetic gene clusters, each goadsporin biosynthetic gene shows low homology to these genes. Goadsporin biosynthesis is initiated by the translation of , and the subsequent cyclization, dehydration and acetylation are probably catalysed by , , , and gene products. shows high similarity to the 54 kDa subunit of the signal recognition particle and plays an important role in goadsporin immunity. Furthermore, four goadsporin analogues were produced by site-directed mutagenesis of , suggesting that this biosynthesis machinery is used for the heterocyclization of peptides.

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2005-12-01
2019-10-15
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