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Abstract

Sequence types of pneumococci isolated in Scotland between 1996 and 2003 were compared with those of globally prevalent antibiotic-resistant clones. Multilocus sequence typing was performed on 252 invasive pneumococcal isolates referred to the Scottish Meningococcus and Pneumococcus Reference Laboratory. Isolates were not preselected for antimicrobial resistance, patient age or disease caused. Sequence types were compared with globally significant antimicrobial-resistant clones identified by the Pneumococcal Molecular Epidemiology Network (PMEN). Sequence types identical with three of the 26 PMEN clones were present in the Scottish collection; the clones were the Spain-3 clone (sequence type 156, seven isolates), the England-9 clone (sequence type 9, eight isolates) and the Utah-24 clone (sequence type 377, one isolate). Many Scottish isolates related to PMEN clones had lower antimicrobial MICs than those described for the corresponding PMEN type strain. A number of single- (SLVs) and double-locus variants (DLVs) were present. Fifteen SLVs related to PMEN sequence types 37, 67, 90, 81, 156, 236 and 377 were detected. The collection contained 10 DLVs related to PMEN sequence types 37, 156, 173 and 338. The majority of SLVs and DLVs were penicillin- or erythromycin-sensitive variants of the resistant PMEN type strains. Capsule switching in isolates related to the PMEN clones was also detected. The highest levels of penicillin resistance were detected in sequence type 320 (serotype 19F), which is not a PMEN clone. These data suggest that PMEN clones are not widely distributed in disease-causing isolates in Scotland.

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2006-02-01
2024-12-13
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References

  1. Amezaga M. R, Carter P. E, Cash P, McKenzie H. 2002; Molecular epidemiology of erythromycin resistance in Streptococcus pneumoniae isolates from blood and non-invasive sites. J Clin Microbiol 40:3313–3318 [CrossRef]
    [Google Scholar]
  2. Beall B, McEllistrem M. C, Gertz R. E, Boxrud D. J, Besser J. M, Harrison L. H, Jorgensen J. H, Whitney C. G. Active Bacterial Core Surveillance/Emerging Infections Program Network 2002; Emergence of a novel penicillin-nonsusceptible, invasive serotype 35B clone of Streptococcus pneumoniae within the United States. J Infect Dis 186:118–122 [CrossRef]
    [Google Scholar]
  3. Clarke S. C, Denham B. C, Christie P, Edwards G. F. S. 2003; Pneumococcal disease in Scotland 2000. SCIEH Weekly Report 37:128–129
    [Google Scholar]
  4. Coffey T. J, Berron S, Daniels M, Garcia-Leoni M. E, Cercenado E, Bouza E, Fenoll A, Spratt B. G. 1996; Multiply antibiotic-resistant Streptococcus pneumoniae recovered from Spanish hospitals (1988–1994): novel major clones of serotypes 14, 19F and 15F. Microbiology 142:2747–2757 [CrossRef]
    [Google Scholar]
  5. Denham B. C, Clarke S. C. 2005; Serotype incidence and antibiotic susceptibility of Streptococcus pneumoniae causing invasive disease in Scotland, 1999–2002. J Med Microbiol 54:327–331 [CrossRef]
    [Google Scholar]
  6. Enright M. C, Spratt B. G. 1998a; A multilocus sequence typing scheme for Streptococcus pneumoniae : identification of clones associated with serious invasive disease. Microbiology 144:3049–3060 [CrossRef]
    [Google Scholar]
  7. Enright M. C, Spratt B. G. 1998b; Extensive variation in the ddl gene of penicillin-resistant Streptococcus pneumoniae results from a hitchhiking effect driven by the pencillin-binding protein 2b gene. Mol Biol Evol 16:1687–1695
    [Google Scholar]
  8. Jefferies J, Clarke S, Diggle M, Smith A, Dowson C, Mitchell T. 2003; Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer. Mol Biotechnol 24:303–307 [CrossRef]
    [Google Scholar]
  9. Jefferies J. M. C, Smith A, Clarke S. C, Dowson C, Mitchell T. J. 2004; Genetic analysis of diverse disease causing pneumococci indicates high levels of diversity within serotypes and capsule switching. J Clin Microbiol 42:5681–5688 [CrossRef]
    [Google Scholar]
  10. Kislak J. W, Razavi L. M. B, Daly A. K, Finland M. 1965; Susceptibility of pneumococci to nine antibiotics. Am J Med Sci 250:261–268 [CrossRef]
    [Google Scholar]
  11. Klugman K. P. 1990; Pneumococcal resistance to antibiotics. Clin Microbiol Rev 3:171–196
    [Google Scholar]
  12. Kyaw M. H, Clarke S. C, Edwards G. F. S, Jones I. G, Campbell H. 2000; Serotypes/groups distribution and antimicrobial resistance of invasive pneumococcal isolates: implications for vaccine strategies. Epidemiol Infect 125:561–572 [CrossRef]
    [Google Scholar]
  13. Kyaw M. H, Clarke S. C, Jones I. G, Campbell H. 2002; Incidence of invasive pneumococcal disease in Scotland, 1988–1999. Epidemiol Infect 128:139–147
    [Google Scholar]
  14. McDougal L. K, Rasheed J. K, Biddle J. W, Tenover F. C. 1995; Identification of multiple clones of extended-spectrum cephalosporin-resistant Streptococcus pneumoniae isolates in the United States. Antimicrob Agents Chemother 39:2282–2288 [CrossRef]
    [Google Scholar]
  15. McGee L, McDougal L, Zhou J. 8 other authors 2001; Nomenclature of major antimicrobial-resistant clones of Streptococcus pneumoniae defined by the pneumococcal molecular epidemiology network. J Clin Microbiol 39:2565–2571 [CrossRef]
    [Google Scholar]
  16. Munoz R, Musser M, Crain M, Briles D. E, Marton A, Parkinson A. J, Sorensen U, Tomasz A. 1992; Geographic distribution of penicillin-resistant clones of Streptococcus pneumoniae : characterised by penicillin-binding protein profile, surface protein A typing and multi locus enzyme analysis. Clin Infect Dis 15:112–118 [CrossRef]
    [Google Scholar]
  17. Schreiber J, Jacobs M. 1995; Antibiotic-resistant pneumococci. Pediatr Clin North Am 42:519–537
    [Google Scholar]
  18. Smart L. E. 1986; Serotyping of Streptococcus pneumoniae strains by coagglutination. J Clin Pathol 39:328–331 [CrossRef]
    [Google Scholar]
  19. Smith A. M, Klugman K. P. 1997; Three predominant clones identified within penicillin-resistant South African isolates of Streptococcus pneumoniae . Microb Drug Resist 3:385–389 [CrossRef]
    [Google Scholar]
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