1887

Abstract

is the most thoroughly studied species of a new bacterial phylogenetic group designated the phylum Acidobacteria. Through a search, the gene has been identified, and its product purified. Electrophoretic mobility shift assays have shown that LexA protein binds specifically to the direct repeat GTTCNGTTC motif. Strikingly, this is also the LexA box of the Alphaproteobacteria, but had not previously been described outside this subclass of the Proteobacteria. In addition, a phylogenetic analysis of the LexA protein clusters together and the Alphaproteobacteria, moving the latter away from their established phylogenetic position as a subclass of the Proteobacteria, and pointing to a lateral gene transfer of the gene from the phylum Acidobacteria, or an immediate ancestor, to the Alphaproteobacteria. Lastly, experiments demonstrate that the gene is DNA-damage inducible, despite the fact that a LexA-binding sequence is not present in its promoter region.

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2006-04-01
2019-11-19
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