1887

Abstract

is an oral pathogen that causes aggressive periodontitis as well as sometimes life-threatening, extra-oral infections. Iron regulation is thought to be important in the pathogenesis of infections and, consistent with this hypothesis, the gene has recently been identified and characterized in . In this study, 14 putatively Fur-regulated genes were identified by Fur titration assay (Furta) in , including , , , , , and – some of which are known to be Fur regulated in other species. A mutant strain was created by selecting for manganese resistance in order to study the Fur regulon. Comparisons between the gene sequences revealed that nucleotide 66 changed from C in the wild-type to T in the mutant strain, changing leucine to isoleucine. The mutant strain expressed a nonfunctional Fur protein as determined by -based ferric uptake assays and Western blotting. It was also more sensitive to acid stress and expressed higher levels of than the wild-type strain. , which inhibits cell division in other bacterial species and whose regulation by iron has not been previously described, was found to be Fur regulated in by Furta, by gel shift assays, and by RT-qPCR assays for gene expression.

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2006-03-01
2020-09-27
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