1887

Abstract

Phospholipases C are known to be important regulators of cellular processes but may also act as virulence factors of pathogenic microbes. At least three genes in the genome of the human-pathogenic fungus encode phospholipases with conserved phospholipase C (Plc) motifs. None of the deduced protein sequences contain N-terminal signal peptides, suggesting that these phospholipases are not secreted. In contrast to its orthologue in , seems to be an essential gene. However, a conditional mutant with reduced transcript levels of had phenotypes similar to Plc1p-deficient mutants in , including reduced growth on media causing increased osmotic stress, on media with a non-glucose carbon source, or at elevated or lower temperatures, suggesting that CaPlc1p, like the Plc1p counterpart in , may be involved in multiple cellular processes. Furthermore, phenotypic screening of the heterozygous Δ/ mutant showed additional defects in hyphal formation. The loss of cannot be compensated by two additional genes of ( and ) encoding two almost identical phospholipases C with no counterpart in but containing structural elements found in bacterial phospholipases C. Although the promoter sequences of and differed dramatically, the transcriptional pattern of both genes was similar. In contrast to , and are not essential. Although / mutants had reduced abilities to produce hyphae on solid media, these mutants were as virulent as the wild-type in a model of systemic infection. These data suggest that contains two different classes of phospholipases C which are involved in cellular processes but which have no specific functions in pathogenicity.

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2005-10-01
2019-11-12
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