1887

Abstract

PerR is one of the most important transcriptional regulators involved in the oxidative-stress response in . Here, the homologous gene in , ranked among the leading causes of nosocomial infection, was characterized and analysed. Phenotype analysis showed that the mutant was significantly more resistant to HO challenge (<0·05). Expression of eight genes with potential roles in the oxidative-stress response was determined in the wild-type and -mutant strains by real-time quantitative PCR. Surprisingly, low quantitative differences in the transcriptional activity of these genes in the mutant versus wild-type were observed. Likewise, this locus was not involved in survival within murine macrophages, but in the mouse peritonitis model, the mutant appeared less lethal than the JH2-2 wild-type strain. The combined results show that PerR affects virulence and that its implication in the transcriptional regulation in this bacterium deviates from the model.

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2005-12-01
2020-07-10
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