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Volume 152, Issue 1

Molecular analysis of the glucose-specific phosphoenolpyruvate : sugar phosphotransferase system from and its links with the control of sugar metabolism Free

Abstract

transports glucose preferentially by a mannose-class phosphoenolpyruvate : sugar phosphotransferase system (PTS). The genomic analysis of allowed the authors to find a gene cluster () encoding the IIAB (), IIC () and IID () proteins of a mannose-class PTS, and a putative 121 aa protein of unknown function (encoded by ), homologues of which are also present in clusters that encode glucose/mannose transporters in other Gram-positive bacteria. The operon is constitutively expressed into a messenger, but an additional transcript was also detected. Upstream of the operon, two genes ( and ) were found which encode proteins resembling a transcriptional regulator and a membrane protein, respectively. Disruption of either or did not affect transcription, and had no effect on glucose uptake. Cells carrying a deletion transported glucose at a rate similar to that of the wild-type strain. By contrast, a disruption resulted in cells unable to transport glucose by the PTS, thus confirming the functional role of the genes. In addition, the mutant exhibited neither inducer exclusion of maltose nor glucose repression. This result confirms the need for glucose transport through the PTS to trigger these regulatory processes in .

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  • Accepted:
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  • Published Online:
Keyword(s): 2-DG, 2-deoxyglucose , CCR, carbon catabolite repression , LAB, lactic acid bacteria , PTS, phosphoenolpyruvate : sugar phosphotransferase system and RACE, rapid amplification of cDNA ends
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2006-01-01
2024-04-23
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Molecular analysis of the glucose-specific phosphoenolpyruvate : sugar phosphotransferase system from Lactobacillus casei and its links with the control of sugar metabolism
Microbiology 152, 95 (2006); https://doi.org/10.1099/mic.0.28293-0
/content/journal/micro/10.1099/mic.0.28293-0
/content/journal/micro/10.1099/mic.0.28293-0
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