RT Journal Article SR Electronic(1) A1 Miyazaki, Kohji A1 Hirase, Tatsuaki A1 Kojima, Yoichi A1 Flint, Harry JamesYR 2005 T1 Medium- to large-sized xylo-oligosaccharides are responsible for xylanase induction in Prevotella bryantii B14 JF Microbiology, VO 151 IS 12 SP 4121 OP 4125 DO https://doi.org/10.1099/mic.0.28270-0 PB Microbiology Society, SN 1465-2080, AB Experiments were done to define the nature of the xylan-derived induction signal for xylanase activity, and evaluate which xylanase genes among the three known ones (xynA, xynB and xynC) are induced by the presence of xylan in Prevotella bryantii B14. During the later stages of exponential growth on glucose, addition of 0·05 % water-soluble xylan (WS-X) stimulated xylanase formation within 30 min. Xylose, xylobiose, xylotriose, xylotetraose, xylopentaose, arabinose and glucuronic acid all failed to induce the xylanase activity. An acid-ethanol-soluble fraction of WS-X (approximate degree of polymerization 30) enhanced the activity significantly, whereas the acid-ethanol-insoluble fraction had no effect, unless first digested by the cloned P. bryantii XynC xylanase. These results indicate that medium- to large-sized xylo-oligosaccharides are responsible for induction. The transcription of all three known xylanase genes from P. bryantii was upregulated coordinately by addition of WS-X. There have been relatively few investigations into the regulation of xylanase activity in bacteria, and it appears to be unique that medium- to large-sized xylo-oligosaccharides are responsible for induction., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.28270-0