1887

Abstract

A triglyceride lipase gene was identified in the genome of strain PH-1. The predicted protein encoded by contains 591 amino acid residues with a putative N-terminal signal peptide and shows 57 and 40–44 % identity to a lipase and five lipases, respectively. Yeast cells overexpressing LIP1 showed lipolytic activity against a broad range of triglyceride substrates. Northern blot analyses revealed that expression of was activated during the fungal infection process. expression was strongly induced in minimal medium supplemented with wheatgerm oil, but only weakly induced by olive oil and triolein. In contrast, supplementation with other carbon sources, including glucose, sucrose, apple pectin and wheat cell-wall material, did not induce expression. Saturated fatty acids were the strongest inducers for expression and this induction was suppressed proportionally by the presence of the unsaturated fatty acid. To determine the potential function of , gene replacement was conducted on strain PH-1. When compared with wild-type PH-1, Δ mutants showed greatly reduced lipolytic activities at the early stage of incubation on minimal medium supplemented with either saturated or unsaturated lipid as the substrate, indicating that encodes a secreted lipase for exogenous lipid hydrolysis. Moreover, the Δ mutants exhibited growth deficiency on both liquid and solid minimal media supplemented with the saturated triglyceride tristearin as the sole carbon source, suggesting that is required for utilization of this substance. Despite these differences, no variation in disease symptoms between the Δ mutants and the wild-type strain was observed on susceptible cereal hosts.

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2005-12-01
2019-10-17
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