1887

Abstract

The genes are necessary for maintaining the outer-membrane integrity of Gram-negative bacteria. These genes were first described in , and more recently in several other species. They are involved in the pathogenesis of , , and . The role of the genes in bacterial pathogenesis was investigated in the phytopathogenic enterobacterium , assuming that this organism might be a good model for such a study. The whole region was characterized. Tol-Pal proteins, except TolA, showed high identity scores with their homologues. mutants were constructed by introducing a –kan cassette in the , , , , and genes. All the mutants were hypersensitive to bile salts. Mutations in , , and were deleterious for the bacteria, which required high concentrations of sugars or osmoprotectants for their viability. Consistent with this observation, they were greatly impaired in their cell morphology and division, which was evidenced by observations of cell filaments, spherical forms, membrane blebbing and mislocalized bacterial septa. Moreover, mutants showed a reduced virulence in a potato tuber model and on chicory leaves. This could be explained by a combination of impaired phenotypes in the mutants, such as reduced growth and motility and a decreased production of pectate lyases, the major virulence factor of .

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2005-10-01
2020-10-24
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