1887

Abstract

sp. strain DBF63 is capable of degrading fluorene (FN) to tricarboxylic acid cycle intermediates via phthalate and protocatechuate. Genes were identified for the protocatechuate branch of the -ketoadipate pathway (, ) by sequence analysis of a 70 kb DNA region of the FN-catabolic linear plasmid pDBF1. RT-PCR analysis of RNA from DBF63 cells grown with FN, dibenzofuran, and protocatechuate indicated that the operon was expressed during both FN and protocatechuate degradation in strain DBF63. The gene encoding -ketoadipate enol-lactone hydrolase () was not fused to the next gene, which encodes -carboxymuconolactone decarboxylase (), in strain DBF63, even though the presence of the gene (the fusion of and ) within a gene cluster has been thought to be a Gram-positive trait. Quantitative RT-PCR analysis revealed that mRNA levels increased sharply in response to protocatechuate, and a biotransformation experiment with ,-muconate using carrying both and indicated that PcaD exhibited -ketoadipate enol-lactone hydrolase activity. The location of the gene cluster on the linear plasmid, and the insertion sequences around the gene cluster suggest that the ecologically important -ketoadipate pathway genes, usually located chromosomally, may be spread widely among bacterial species via horizontal transfer or transposition events.

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2005-11-01
2020-04-09
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