1887

Abstract

is a pathogen of rodents, which causes diverse enteric and hepatic inflammatory diseases and malignancies. The urease enzyme is an important colonization factor of gastric species like , but little is known about the role and regulation of urease in enterohepatic species. Here it is reported that urease activity of does not contribute to acid resistance, and that it is nickel-responsive at the post-translational level. strain ATCC 51449 did not grow or survive at pH 3·0, and supplementation with urea or NiCl did not abrogate this acid sensitivity. Furthermore, urease enzyme activity of was acid-independent, which contrasts with the acid-induced urease system of . Nickel supplementation of Brucella medium resulted in a tenfold increase in urease activity in both and , but the maximum level of urease activity in was still three- to fivefold lower when compared to in the same conditions. The increase in urease activity of was not associated with elevation of urease mRNA or protein levels. Inhibition of protein synthesis by chloramphenicol did not affect nickel-responsive induction of urease activity in , and confirmed that nickel induction occurs at the post-translational level, probably by activation of preformed apo-enzyme. In conclusion, both the role of the urease enzyme and the regulation of urease activity differ between the enterohepatic pathogen and the gastric pathogen .

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2005-12-01
2020-08-09
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