1887

Abstract

The authors previously reported that interspecific stimulatory events between species for antibiotic production and/or morphological differentiation mediated by putative diffusible metabolites take place at a high frequency. This paper reports the isolation and characterization of a substance produced by that stimulates the growth and development of . The substance was purified from the culture supernatant of by using anion-exchange chromatography, gel filtration chromatography and reverse-phase HPLC. FAB-MS and NMR analyses of the purified preparation indicated the substance to be desferrioxamine E (synonym: nocardamine), a siderophore that is widely produced by species and related organisms. Similar stimulatory effects on the growth and development of were exerted by desferrioxamine E produced by another actinomycete strain, but not by other siderophores tested, including ferrichrome and nocobactin and free ferric ion. An exogenous supply of desferrioxamine E stimulated secondary metabolite formation and/or morphological differentiation in various actinomycete strains. Disruption of the desferrioxamine biosynthesis gene cluster in A3(2) abolished the production of desferrioxamine E and the activity to stimulate the growth and differentiation of . The mutant showed impaired growth and development on Bennett's/glucose agar medium, but it was rescued by the exogenous supply of desferrioxamine E. These results indicate that desferrioxamines play an important role in streptomycete physiology. Similar to several pathogenic bacteria and fungi, may be defective in the production of siderophores; however, it can utilize the siderophores excreted by other organisms.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.28139-0
2005-09-01
2020-04-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/151/9/mic1512899.html?itemId=/content/journal/micro/10.1099/mic.0.28139-0&mimeType=html&fmt=ahah

References

  1. Barona-Gomez F., Wong U., Giannakopulos A. E., Derrick P. J., Challis G. L. 2004; Identification of a cluster of genes that directs desferrioxamine biosynthesis in Streptomyces coelicolor M145. J Am Chem Soc126:16282–16283[CrossRef]
    [Google Scholar]
  2. Beck E., Ludwig G., Auerswald E. A., Reiss B., Schaller H. 1982; Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn 5. Gene19:327–336[CrossRef]
    [Google Scholar]
  3. Bentley S. D., Chater K. F., Cerdeno-Tarraga A. M.. 40 other authors 2002; Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2. Nature417:141–147[CrossRef]
    [Google Scholar]
  4. Berner I., Konetschny-Rapp S., Jung G., Winkelmann G. 1988; Characterization of ferrioxamine E as the principal siderophore of Erwinia herbicola ( Enterobacter agglomerans ). Biol Met1:51–56[CrossRef]
    [Google Scholar]
  5. Challis G. L., Ravel J. 2000; Coelichelin, a new peptide siderophore encoded by the Streptomyces coelicolor genome: structure prediction from the sequence of its non-ribosomal peptide synthetase. FEMS Microbiol Lett187:111–114[CrossRef]
    [Google Scholar]
  6. Chater K. F. 1993; Genetics of differentiation in Streptomyces . Annu Rev Microbiol47:685–713[CrossRef]
    [Google Scholar]
  7. Horinouchi S. 1999; Gamma-butyrolactones that control secondary metabolism and cell differentiation in Streptomyces . In Cell–Cell Signaling in Bacteria pp193–207 Edited by Dunny G. M., Winans S. C.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Horinouchi S. 2002; A microbial hormone, A-factor, as a master switch for morphological differentiation and secondary metabolism in Streptomyces griseus . Front Biosci7:2045–2057[CrossRef]
    [Google Scholar]
  9. Horinouchi S., Beppu T. 1992; Autoregulatory factors and communication in actinomycetes. Annu Rev Microbiol46:377–398[CrossRef]
    [Google Scholar]
  10. Horinouchi S., Kumada Y., Beppu T. 1984; Unstable genetic determinant of A-factor biosynthesis in streptomycin-producing organisms: cloning and characterization. J Bacteriol158:481–487
    [Google Scholar]
  11. Kieser T., Bibb M. J., Buttner M. J., Chater K. F., Hopwood D. A. 2000; Practical Streptomyces Genetics Norwich, UK: John Innes Foundation;
    [Google Scholar]
  12. Kingsley R. A., Reissbrodt R., Rabsch W.. 7 other authors 1999; Ferrioxamine-mediated iron(III) utilization by Salmonella enterica . Appl Environ Microbiol65:1610–1618
    [Google Scholar]
  13. Lesuisse E., Blaiseau P. L., Dancis A., Camadro J. M. 2001; Siderophore uptake and use by the yeast Saccharomyces cerevisiae . Microbiology147:289–298
    [Google Scholar]
  14. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Matsubara K., Sakuda S., Kondo T., Tanaka M., Nishimura T., Suzuki A. 1998; Morphological changes in insect BM-N4 cells induced by nocardamine. Biosci Biotechnol Biochem62:2049–2051[CrossRef]
    [Google Scholar]
  16. Meyer J. M., Abdallah M. A. 1980; The siderochromes of nonfluorescent Pseudomonas : production of nocardamine by Pseudomonas stutzeri . J Gen Microbiol118:125–129
    [Google Scholar]
  17. Miyadoh S. 1993; Research on antibiotic screening in Japan over the last decade: a producing microorganisms approach. Actinomycetologica7:100–106[CrossRef]
    [Google Scholar]
  18. Müller G., Raymond K. N. 1984; Specificity and mechanism of ferrioxamine-mediated iron transport in Streptomyces pilosus . J Bacteriol160:304–312
    [Google Scholar]
  19. Müller G., Matzanke B. F., Raymond K. N. 1984; Iron transport in Streptomyces pilosus mediated by ferrichrome siderophores, rhodotorulic acid, and enantio-rhodotorulic acid. J Bacteriol160:313–318
    [Google Scholar]
  20. Prelog V. 1963; Iron containing antibiotics and microbic growth factors. Pure Appl Chem6:327–338
    [Google Scholar]
  21. Schryvers A. B., Stojiljkovic I. 1999; Iron acquisition systems in the pathogenic Neisseria. Mol Microbiol32:1117–1123[CrossRef]
    [Google Scholar]
  22. Schupp T., Waldmeier U., Divers M. 1987; Biosynthesis of desferrioxamine B in Streptomyces pilosus : evidence for the involvement of lysine decarboxylase. FEMS Microbiol Lett42:135–139[CrossRef]
    [Google Scholar]
  23. Schwyn B., Neilands J. B. 1987; Universal chemical assay for the detection and determination of siderophores. Anal Biochem160:47–56[CrossRef]
    [Google Scholar]
  24. Takano E., Nihira T., Hara Y., Jones J. J., Gershater C. J., Yamada Y., Bibb M. 2000; Purification and structural determination of SCB1, a gamma-butyrolactone that elicits antibiotic production in Streptomyces coelicolor A3(2. J Biol Chem275:11010–11016[CrossRef]
    [Google Scholar]
  25. Tam T. F., Leung-Toung R., Li W., Wang Y., Karimian K., Spino M. 2003; Iron chelator research: past, present, and future. Curr Med Chem10:983–995[CrossRef]
    [Google Scholar]
  26. Ueda K., Kawai S., Ogawa H., Kiyama A., Kubota T., Kawanobe H., Beppu T. 2000; Wide distribution of interspecific stimulatory events on antibiotic production and sporulation among Streptomyces species. J Antibiot53:979–982[CrossRef]
    [Google Scholar]
  27. Yamada Y. 1999; Autoregulatory factors and regulation of antibiotic production in Streptomyces . In Microbial Signalling and Communication pp177–196 Edited by England R. R., Hobbs G., Bainton N. J., Roberts D. M.. Cambridge: Cambridge University Press;
    [Google Scholar]
  28. Yun C. W., Ferea T., Rashford J., Ardon O., Brown P. O., Botstein D., Kaplan J., Philpott C. C. 2000; Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae . Evidence for two pathways of iron uptake. J Biol Chem275:10709–10715[CrossRef]
    [Google Scholar]
  29. Zahner H., Bachmann E., Hutter R., Nuesch J. 1962; Sideramine, eisenhaltige Wachstumsfaktoren aus Mikroorganismen. Pathol Microbiol25:708–736
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.28139-0
Loading
/content/journal/micro/10.1099/mic.0.28139-0
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error