1887

Abstract

The gene cluster, expressed by uropathogenic strains, determines bacterial attachment and invasion. The Dr fimbrial structures formed at the bacterial cell surface are composed of DraE subunits. The Dr fimbriae-coding cluster contains six open reading frames – , , , , and – among which the gene encodes the structural fimbrial subunit DraE. Very little is known about surface expression of the gene product. The expression of DraD and its role in the biogenesis of Dr fimbriae were determined by constructing mutants in the operon and by immunoblot and immunofluorescence experiments. In this study, DraD was found to be a surface-exposed protein. The expression of DraD was independent of the DraC usher and DraE fimbrial subunits. Polymerization of DraE fimbrial subunits into fimbrial structures did not require expression of the DraD protein.

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2005-07-01
2019-10-16
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