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Volume 151, Issue 11

The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in strain MH-110 Free

Abstract

MH-110 possesses three different sets of genes for ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO): two form I ( and ) and one form II (). We have previously shown that the expression of these RubisCO genes is dependent on the ambient CO concentration. LysR-type transcriptional regulators, designated CbbR1 and CbbRm, are encoded upstream of the and genes, respectively. In this study, we revealed by gel shift assay that CbbR1 and CbbRm bind with higher affinity to the promoter regions of and , respectively, and with lower affinity to the other RubisCO gene promoters. The expression patterns of the three RubisCOs in the and the gene mutants showed that CbbR1 and CbbRm were required to activate the expression of and , respectively, and that neither CbbR1 nor CbbRm was required for the expression of . The expression of was significantly enhanced under high-CO conditions in the mutant, in which the expression of was decreased. Although was not expressed under high-CO conditions in the wild-type strain or the single mutants, the expression of was observed in the double mutant, in which the expression of both and was decreased. These results indicate that there is an interactive regulation among the three RubisCO genes.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): CBB cycle, Calvin–Benson–Bassham cycle , LTTR, LysR-type transcriptional regulator , PEP, phosphoenolpyruvate and RubisCO, d-ribulose 1,5-bisphosphate carboxylase/oxygenase
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2005-11-01
2024-04-20
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The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in Hydrogenovibrio marinus strain MH-110
Microbiology 151, 3615 (2005); https://doi.org/10.1099/mic.0.28056-0
/content/journal/micro/10.1099/mic.0.28056-0
/content/journal/micro/10.1099/mic.0.28056-0
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