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Volume 151, Issue 8

Stability of the PCC 7942 circadian clock under directed anti-phase expression of the genes Free

Abstract

The , and genes encode the core components of the cyanobacterial circadian clock in PCC 7942. Rhythmic expression patterns of and of the operon normally peak in synchrony. In some mutants the relative timing of peaks (phase relationship) between these transcription units is altered, but circadian rhythms persist robustly. In this study, the importance of the transcriptional timing of genes was examined. Expressing either or from a heterologous promoter whose peak expression occurs 12 h out of phase from the norm, and thus 12 h out of phase from the other locus, did not affect the time required for one cycle (period) or phase of the circadian rhythm, as measured by bioluminescence reporters. Furthermore, the data confirm that specific elements within the promoters of the genes are not necessary to sustain clock function.

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): LD, light/dark and LL, constant light
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2005-08-01
2024-04-18
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Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes
Microbiology 151, 2605 (2005); https://doi.org/10.1099/mic.0.28030-0
/content/journal/micro/10.1099/mic.0.28030-0
/content/journal/micro/10.1099/mic.0.28030-0
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