1887

Abstract

A gene, , encoding a protein similar to the response regulators of bacterial two-component signal transduction systems, was identified upstream of the polyketide gene cluster involved in biosynthesis of the angucycline-like antibiotic auricin in CCM 3239. Expression of the gene was directed by a single promoter, , which was transcribed at low levels during the exponential phase and induced just before the stationary phase. A divergently transcribed gene, , has been identified upstream of , encoding a protein homologous to transcriptional repressors of the TetR family. The gene was disrupted in the CCM 3239 chromosome by homologous recombination. The mutation in the gene had no effect on growth and differentiation. However, biochromatographic analysis of culture extracts from the -disrupted strain revealed that auricin was not produced in the mutant. This indicated that is essential for auricin production. Transcription from the previously characterized promoter, directing expression of the first gene, , in the auricin gene cluster, was dramatically decreased in the CCM 3239 mutant strain. Moreover, the Aur1P protein, overproduced in , was shown to bind specifically upstream of the promoter region. The results indicated that the Aur1P regulator activates expression of the auricin biosynthesis genes.

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2005-08-01
2019-10-23
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