@article{mbs:/content/journal/micro/10.1099/mic.0.28011-0, author = "Prasad, Sathish and Morris, Peter C. and Hansen, Rasmus and Meaden, Philip G. and Austin, Brian", title = "A novel bacteriocin-like substance (BLIS) from a pathogenic strain of Vibrio harveyi", journal= "Microbiology", year = "2005", volume = "151", number = "9", pages = "3051-3058", doi = "https://doi.org/10.1099/mic.0.28011-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.28011-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "BLIS, bacteriocin-like inhibitory substance", keywords = "DLPA, double-layer plate assay", keywords = "2-DE, two-dimensional gel electrophoresis", keywords = "CFS, cell-free supernatant", keywords = "MALDI-TOF-MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry", abstract = "Inter-strain and inter-species inhibition mediated by a bacteriocin-like inhibitory substance (BLIS) from a pathogenic Vibrio harveyi strain VIB 571 was demonstrated against four isolates of the same species, and one culture each of a Vibrio sp., Vibrio fischeri, Vibrio gazogenes and Vibrio parahaemolyticus. The crude BLIS, which was obtained by ammonium-sulphate precipitation of the cell-free supernatant of a 72 h broth culture of strain VIB 571, was inactivated by lipase, proteinase K, pepsin, trypsin, pronase E, SDS and incubation at ≥60 °C for 10 min. The activity was stable between pH 2–11 for at least 5 h. Anion-exchange chromatography, gel filtration, SDS-PAGE and two-dimensional gel electrophoresis revealed the presence of a single major peak, comprising a protein with a pI of ∼5·4 and a molecular mass of ∼32 kDa. The N-terminal amino acid sequence of the protein comprised Asp-Glu-Tyr-Ile-Ser-X-Asn-Lys-X-Ser-Ser-Ala-Asp-Ile (with X representing cysteine or modified amino acid residues). A similarity search based on the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) generated peptide masses and the N-terminal sequence did not yield any significant matches.", }