1887

Abstract

is a Gram-negative enteric bacterium affecting both animals and humans. Recently, a type III secretion system (TTSS) was found in . Such systems are generally used by bacterial pathogens to deliver virulence factors into host cells to subvert normal cell functions. Genome-walking was performed from the and genes (homologues of and , respectively) identified in previous studies, to determine the sequences of the TTSS. Thirty-five ORFs were identified which encode the TTSS apparatus, chaperones, effectors and regulators. Mutants affected in genes representing each category were generated and found to have decreased survival and growth in fish phagocytes. LD values of the mutants were increased by at least 10-fold in comparison to those of the wild-type strain. The adherence and invasion rates of the and mutants were enhanced while those of the other mutants remained similar to the wild-type. The and mutants showed slight autoaggregation in Dulbecco's Modified Eagle Medium, whereas the rest of the mutants failed to autoaggregate. Regulation of the TTSS was found to involve the two-component regulatory system . This study showed that the TTSS is important for pathogenesis. An understanding of this system will provide greater insight into the virulence mechanisms of this bacterial pathogen.

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2005-07-01
2020-07-06
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