1887

Abstract

Phosphoprotein phosphatases encoded by the and genes function in signal transduction pathways for degradation of misfolded proteins in the extracytoplasmic compartments of . In order to trace the evolution of genes and assess their roles in other enteric pathogens, the structure and distribution of these genes among closely related Shigella subgroups were studied. PCR amplification, probe hybridization studies and DNA sequencing were used to determine the genotypes of 58 strains from the four Shigella subgroups, Dysenteriae, Boydii, Sonnei and Flexneri. It was found that the alleles among Shigella subgroups were extremely susceptible to gene inactivation and that the mutations involved in allele inactivation were varied. They included IS insertions, gene replacement by an IS element, a small deletion within the gene or large deletion engulfing the entire gene region, and base substitutions that generated premature termination codons. As a result, of 58 strains studied, only eight (14 %) possessed intact and genes. Of the Shigella strains examined, 76 % (44/58) showed at least one of the alleles inactivated by one or more IS elements, including IS, IS, IS and IS. Phylogenetic analysis revealed that IS elements have been independently acquired in multiple lineages of Shigella, suggesting that loss of functional alleles has been advantageous during Shigella strain evolution.

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2005-08-01
2020-04-09
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