1887

Abstract

The secreted protein pattern of depends on the carbon source present in the culture media. One protein that shows the most dramatic change is the high-affinity phosphate-binding protein PstS, which is strongly accumulated in the supernatant of liquid cultures containing high concentrations (>3 %) of certain sugars, such as fructose, galactose and mannose. The promoter region of this gene and that of its homologue were used to drive the expression of a xylanase in that was accumulated in the culture supernatant when grown in the presence of fructose. PstS accumulation was dramatically increased in a polyphosphate kinase null mutant (Δ) and was impaired in a deletion mutant lacking , the transcriptional regulator gene of the two-component system that controls the Pho regulon. Deletion of the genes in and impaired phosphate transport and accelerated differentiation and sporulation on solid media. Complementation with a single copy in a null mutant returned phosphate transport and sporulation to levels similar to those of the wild-type strain. The present work demonstrates that carbon and phosphate metabolism are linked in the regulation of genes and that this can trigger the genetic switch towards morphogenesis.

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2005-08-01
2019-10-15
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