1887

Abstract

The plasmids from 16 sphingomonads which degrade various xenobiotics and polycyclic aromatic compounds were compared with the previously sequenced plasmid pNL1 from F199. The replicase genes from plasmid pNL1 were amplified by PCR and used as a gene probe for the identification of plasmids belonging to the same incompatibility group as plasmid pNL1. Plasmids were prepared from various sphingomonads and hybridized with the gene probe. Positive hybridization signals were obtained with plasmids of approximately 160–195 kb from and B0695, which had been isolated from the same subsurface location as F199. The probe also hybridized with plasmids from BN6, sp. HH69 and , which had been isolated from different continents and which utilize different organic compounds than F199 and the other subsurface strains. The results of the hybridization experiments were confirmed by PCR experiments using primers deduced from the region of plasmid pNL1. Nucleotide sequence comparisons suggested that three gene clusters were conserved between plasmid pNL1 and plasmid pBN6 from the naphthalenesulfonate- degrading strain BN6. From these sequence comparisons, PCR primers were derived in order to detect the respective gene clusters in the other strains and to deduce their position relative to each other. These experiments demonstrated that all analysed subsurface strains harboured the same three gene clusters, but that the position and distance from each other of the clusters varied considerably among the different strains.

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2005-06-01
2019-11-19
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