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Volume 151, Issue 6

Oral immunization with a mutant of protects against plague Free

Abstract

Inactivation of the gene encoding DNA adenine methylase () has been shown to attenuate some pathogens such as serovar Typhimurium and is a lethal mutation in others such as strain YPIII. In this study the methylase gene in strain IP32953 was inactivated. Unlike the wild-type, DNA isolated from the mutant could be digested with I, which is consistent with an altered pattern of DNA methylation. The mutant was sensitive to bile salts but not to 2-aminopurine. The effect of inactivation on gene expression was examined using a DNA microarray. In BALB/c mice inoculated orally or intravenously with the mutant, the median lethal dose (MLD) was at least 10-fold higher than the MLD of the wild-type. BALB/c mice inoculated with the mutant were protected against a subcutaneous challenge with 100 MLDs of strain GB and an intravenous challenge with 300 MLDs of IP32953.

  • Received:
  • Accepted:
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Keyword(s): 2-AP, 2-aminopurine , i.v., intravenous(ly) , MLD, median lethal dose and s.c., subcutaneous(ly)
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2005-06-01
2022-01-23
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Oral immunization with a dam mutant of Yersinia pseudotuberculosis protects against plague
Microbiology 151, 1919 (2005); https://doi.org/10.1099/mic.0.27959-0
/content/journal/micro/10.1099/mic.0.27959-0
/content/journal/micro/10.1099/mic.0.27959-0
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