1887

Abstract

The catabolic potential for sterol degradation of fast-growing mycobacteria is well known. However, no genes or enzymes responsible for the steroid degradation process have been identified as yet in these species. One of the key enzymes required for degradation of the steroid ring structure is 3-ketosteroid Δ-dehydrogenase (KsdD). The recent annotation of the genome (TIGR database) revealed six KsdD homologues. Targeted disruption of the MSMEG5898 () gene, but not the MSMEG4855 () gene, resulted in partial inactivation of the cholesterol degradation pathway and accumulation of the intermediate 4-androstene-3,17-dione. This effect was reversible by the introduction of the wild-type gene into Δ or overexpression of . The data indicate that KsdD1 is the main KsdD in , but that KsdD2 is able to perform the cholesterol degradation process when overproduced.

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2005-07-01
2020-08-13
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