1887

Abstract

DNA adenine methyltransferase (Dam) plays an important role in physiological processes of Gram-negative bacteria such as mismatch repair and replication. In addition, Dam regulates the expression of virulence genes in various species. The authors cloned the gene of and showed that Dam is essential for viability. Dam overproduction in resulted in an increased frequency of spontaneous mutation and decreased resistance to 2-aminopurine; however, these effects were only marginal compared to the effect of overproduction of -derived Dam in , implying different roles or activities of Dam in mismatch repair of the two species. These differences in Dam function are not the cause for the essentiality of Dam in , as Dam of can complement a defect in . Instead, Dam seems to interfere with expression of essential genes. Furthermore, Dam mediates virulence of . Dam overproduction results in increased tissue culture invasion of , while the expression of specifically -expressed genes is not altered.

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2005-07-01
2020-04-04
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