1887

Abstract

Many bacterial pathogens encode ADP-ribosyltransferase toxins. The authors identified an ADP-ribosyltransferase toxin homologue (ArtA, ArtB) in serovar Typhimurium () DT104. ArtA is most homologous to a putative pertussis-like toxin subunit present in (STY1890) and A (SPA1609), while ArtB shows homology to a hypothetical periplasmic protein of (STY1364) and A (SPA1188), and a putative pertussis-like toxin subunit in (STY1891) and A (SPA1610). The gene was detected from the phage particle fraction upon mitomycin C induction, and the flanking region of contains a prophage-like sequence, suggesting that these putative toxin genes reside within a prophage. Southern blotting analysis revealed that is conserved in 12 confirmed DT104 strains and in four related strains which are not phage-typed but are classified into the same group as DT104 by both amplified-fragment length polymorphism and pulsed-field gel electrophoresis. Except for one strain, NCTC 73, all 13 strains which were classified into different groups from that of DT104 lacked the locus. The results suggest that phage-mediated recombination has resulted in the acquisition of genes in DT104 strains.

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2005-09-01
2020-07-05
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