1887

Abstract

Many bacterial pathogens encode ADP-ribosyltransferase toxins. The authors identified an ADP-ribosyltransferase toxin homologue (ArtA, ArtB) in serovar Typhimurium () DT104. ArtA is most homologous to a putative pertussis-like toxin subunit present in (STY1890) and A (SPA1609), while ArtB shows homology to a hypothetical periplasmic protein of (STY1364) and A (SPA1188), and a putative pertussis-like toxin subunit in (STY1891) and A (SPA1610). The gene was detected from the phage particle fraction upon mitomycin C induction, and the flanking region of contains a prophage-like sequence, suggesting that these putative toxin genes reside within a prophage. Southern blotting analysis revealed that is conserved in 12 confirmed DT104 strains and in four related strains which are not phage-typed but are classified into the same group as DT104 by both amplified-fragment length polymorphism and pulsed-field gel electrophoresis. Except for one strain, NCTC 73, all 13 strains which were classified into different groups from that of DT104 lacked the locus. The results suggest that phage-mediated recombination has resulted in the acquisition of genes in DT104 strains.

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2005-09-01
2019-10-22
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References

  1. Allen, C. A., Fedorka-Cray, P. J., Vazquez-Torres, A., Suyemoto, M., Altier, C., Ryder, L. R., Fang, F. C. & Libby, S. J. ( 2001; ). In vitro and in vivo assessment of Salmonella enterica serovar Typhimurium DT104 virulence. Infect Immun 69, 4673–4677.[CrossRef]
    [Google Scholar]
  2. Anderson, E. S., Ward, L. R., Saxe, M. J. & de Sa, J. D. ( 1977; ). Bacteriophage-typing designations of Salmonella typhimurium. J Hyg 78, 297–300.[CrossRef]
    [Google Scholar]
  3. Arcangioli, M. A., Leroy-Setrin, S., Martel, J. L. & Chaslus-Dancla, E. ( 1999; ). A new chloramphenicol and florfenicol resistance gene flanked by two integron structures in Salmonella typhimurium DT104. FEMS Microbiol Lett 174, 327–332.[CrossRef]
    [Google Scholar]
  4. Bokoch, G. M., Katada, T., Northup, J. K., Hewlett, E. L. & Gilman, A. G. ( 1983; ). Identification of the predominant substrate for ADP-ribosylation by islet activating protein. J Biol Chem 258, 2072–2075.
    [Google Scholar]
  5. Briggs, C. E. & Fratamico, P. M. ( 1999; ). Molecular characterization of an antibiotic resistance gene cluster of Salmonella typhimurium DT104. Antimicrob Agents Chemother 43, 846–849.[CrossRef]
    [Google Scholar]
  6. Burnette, W. N. ( 1994; ). AB5 ADP-ribosylating toxins: comparative anatomy and physiology. Structure 2, 151–158.[CrossRef]
    [Google Scholar]
  7. Carlson, S. A., Meyerholz, D. K., Stabel, T. J. & Jones, B. D. ( 2001; ). Secretion of a putative cytotoxin in multiple antibiotic resistant Salmonella enterica serotype Typhimurium phagetype DT104. Microb Pathog 31, 201–204.[CrossRef]
    [Google Scholar]
  8. Glynn, M. K., Bopp, C., Dewitt, W., Dabney, P., Mokhtar, M. & Angulo, F. J. ( 1998; ). Emergence of multidrug-resistant Salmonella enterica serotype Typhimurium DT104 infections in the United States. N Engl J Med 338, 1333–1338.[CrossRef]
    [Google Scholar]
  9. Ikebe, T., Wada, A., Inagaki, Y. & 8 other authors ( 2002; ). Dissemination of the phage-associated novel superantigen gene speL in recent invasive and noninvasive Streptococcus pyogenes M3/T3 isolates in Japan. Infect Immun 70, 3227–3233.[CrossRef]
    [Google Scholar]
  10. Katada, T. & Ui, M. ( 1982; ). Direct modification of the membrane adenylate cyclase system by islet-activating protein due to ADP-ribosylation of a membrane protein. Proc Natl Acad Sci U S A 79, 3129–3133.[CrossRef]
    [Google Scholar]
  11. McClelland, M., Sanderson, K. E., Spieth, J. & 23 other authors ( 2001; ). Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature 413, 852–856.[CrossRef]
    [Google Scholar]
  12. McClelland, M., Sanderson, K. E., Clifton, S. W. & 32 other authors ( 2004; ). Comparison of genome degradation in Paratyphi A and Typhi, human-restricted serovars of Salmonella enterica that cause typhoid. Nat Genet 36, 1268–1274.[CrossRef]
    [Google Scholar]
  13. Merritt, E. A. & Hol, W. G. ( 1995; ). AB5 toxins. Curr Opin Struct Biol 5, 165–171.[CrossRef]
    [Google Scholar]
  14. Mmolawa, P. T., Willmore, R., Thomas, C. J. & Heuzenroeder, M. W. ( 2002; ). Temperate phages in Salmonella enterica serovar Typhimurium: implications for epidemiology. Int J Med Microbiol 291, 633–644.[CrossRef]
    [Google Scholar]
  15. Mmolawa, P. T., Schmieger, H., Tucker, C. P. & Heuzenroeder, M. W. ( 2003; ). Genomic structure of the Salmonella enterica serovar Typhimurium DT 64 bacteriophage ST64T: evidence for modular genetic architecture. J Bacteriol 185, 3473–3475.[CrossRef]
    [Google Scholar]
  16. Ng, L. K., Mulvey, M. R., Martin, I., Peters, G. A. & Johnson, W. ( 1999; ). Genetic characterization of antimicrobial resistance in Canadian isolates of Salmonella serovar Typhimurium DT104. Antimicrob Agents Chemother 43, 3018–3021.
    [Google Scholar]
  17. Pallen, M. J., Lam, A. C., Loman, N. J. & McBride, A. ( 2001; ). An abundance of bacterial ADP-ribosyltransferases – implications for the origin of exotoxins and their human homologues. Trends Microbiol 9, 302–307.[CrossRef]
    [Google Scholar]
  18. Parkhill, J., Dougan, G., James, K. D. & 38 other authors ( 2001; ). Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18. Nature 413, 848–852.[CrossRef]
    [Google Scholar]
  19. Ridley, A. & Threlfall, E. J. ( 1998; ). Molecular epidemiology of antibiotic resistance genes in multiresistant epidemic Salmonella typhimurium DT 104. Microb Drug Resist 4, 113–118.[CrossRef]
    [Google Scholar]
  20. Sameshima, T., Akiba, M., Izumiya, H., Terajima, J., Tamura, K., Watanabe, H. & Nakazawa, M. ( 2000; ). Salmonella typhimurium DT104 from livestock in Japan. Jpn J Infect Dis 53, 15–16.
    [Google Scholar]
  21. Sandvang, D., Aarestrup, F. M. & Jensen, L. B. ( 1998; ). Characterisation of integrons and antibiotic resistance genes in Danish multiresistant Salmonella enterica Typhimurium DT104. FEMS Microbiol Lett 160, 37–41.[CrossRef]
    [Google Scholar]
  22. Tamada, Y., Nakaoka, Y., Nishimori, K. & 9 other authors ( 2001; ). Molecular typing and epidemiological study of Salmonella enterica serotype Typhimurium isolates from cattle by fluorescent amplified-fragment length polymorphism fingerprinting and pulsed-field gel electrophoresis. J Clin Microbiol 39, 1057–1066.[CrossRef]
    [Google Scholar]
  23. Tanaka, K., Nishimori, K., Makino, S. & 8 other authors ( 2004; ). Molecular characterization of a prophage of Salmonella enterica serotype Typhimurium DT104. J Clin Microbiol 42, 1807–1812.[CrossRef]
    [Google Scholar]
  24. Threlfall, E. J., Frost, J. A., Ward, L. R. & Rowe, B. ( 1994; ). Epidemic in cattle and humans of Salmonella typhimurium DT 104 with chromosomally integrated multiple drug resistance. Vet Rec 134, 577.[CrossRef]
    [Google Scholar]
  25. Threlfall, E. J., Frost, J. A., Ward, L. R. & Rowe, B. ( 1996; ). Increasing spectrum of resistance in multiresistant Salmonella typhimurium. Lancet 347, 1053–1054.
    [Google Scholar]
  26. Villar, R. G., Macek, M. D., Simons, S. & 7 other authors ( 1999; ). Investigation of multidrug-resistant Salmonella serotype Typhimurium DT104 infections linked to raw-milk cheese in Washington State. JAMA 281, 1811–1816.[CrossRef]
    [Google Scholar]
  27. Wagner, P. L. & Waldor, M. K. ( 2002; ). Bacteriophage control of bacterial virulence. Infect Immun 70, 3985–3993.[CrossRef]
    [Google Scholar]
  28. Wu, M. T., Carlson, S. A. & Meyerholz, D. K. ( 2002; ). Cytopathic effects observed upon expression of a repressed collagenase gene present in Salmonella and related pathogens: mimicry of a cytotoxin from multiple antibiotic-resistant Salmonella enterica serotype Typhimurium phagetype DT104. Microb Pathog 33, 279–287.[CrossRef]
    [Google Scholar]
  29. Yee, A. J., De Grandis, S. & Gyles, C. L. ( 1993; ). Mitomycin-induced synthesis of a Shiga-like toxin from enteropathogenic Escherichia coli H.I.8. Infect Immun 61, 4510–4513.
    [Google Scholar]
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