1887

Abstract

Eap and Emp are two adhesins initially described as extracellular matrix binding proteins. Eap has since emerged as being important in adherence to and invasion of eukaryotic cells, as well as being described as an immunomodulator and virulence factor in chronic infections. This paper describes the mapping of the transcription start point of the and promoters. Moreover, using reporter-gene assays and real-time PCR in defined regulatory mutants, environmental conditions and global regulators affecting expression of and were investigated. Marked differences were found in expression of and between strain Newman and the 8325 derivatives SH1000 and 8325-4. Moreover, both genes were repressed in the presence of glucose. Analysis of expression of both genes in various regulatory mutants revealed that and were involved in their regulation, but the data suggested that there were additional regulators of both genes. In a mutant, expression of both genes was severely repressed. expression was also reduced in the presence of glucose, suggesting that repression of and in glucose-containing medium may, in part, be a consequence of a decrease in expression of .

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2005-06-01
2020-04-07
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