1887

Abstract

The methylotrophic yeast was able to grow on pectic compounds, pectin and polygalacturonate, as sole carbon sources. Under the growth conditions used, exhibited increased levels of pectin methylesterase, and pectin-depolymerizing and methanol-metabolizing enzyme activities. On the other hand, has two alcohol oxidase (AOD) genes, and . On growth on pectin, the Δ and ΔΔ strains showed a severe defect in the growth yield, although the Δ strain could grow on polygalacturonate to the same extent as the wild-type strain. The expression of was detected in pectin-grown cells, but the -gene expression detected by pectin was much lower than that of . Moreover, pectin could induce peroxisome proliferation in , like methanol and oleic acid. These findings showed that was able to utilize the methylester moiety of pectin by means of methanol-metabolic enzymes in peroxisomes, and that the functional AOD subunit for pectin utilization was Mod1p in .

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2005-06-01
2020-07-09
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