1887

Abstract

The gene hierarchy directing biogenesis of peritrichous flagella on the surface of and other enterobacteria is controlled by the heterotetrameric master transcriptional regulator FlhDC. To assess the extent to which FlhDC directly activates promoters of a wider regulon, a computational screen of the genome was used to search for gene-proximal DNA sequences similar to the 42–44 bp inverted repeat FlhDC binding consensus. This identified the binding sequences upstream of all eight flagella class II operons, and also putative novel FlhDC binding sites in the promoter regions of 39 non-flagellar genes. Nine representative non-flagellar promoter regions were all bound by active reconstituted FlhDC over the range 38–356 nM, and of the nine corresponding chromosomal promoter– fusions, those of the four genes , , and showed up to 50-fold dependence on FlhDC . In comparison, four representative flagella class II promoters bound FlhDC in the range 12–43 nM and were upregulated 30- to 990-fold. The FlhDC-binding sites of the four regulated non-flagellar genes overlap by 1 or 2 bp the predicted −35 motif of the FlhDC-activated promoters, as is the case with FlhDC-dependent class II flagellar promoters. The data indicate a wider FlhDC regulon, in which non-flagellar genes are bound and activated directly, albeit less strongly, by the same mechanism as that regulating the flagella gene hierarchy.

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2005-06-01
2019-10-16
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