1887

Abstract

The biofilm-associated protein (Bap) is a surface protein implicated in biofilm formation by isolated from chronic mastitis infections. The gene is carried in a putative composite transposon inserted in SaPIbov2, a mobile staphylococcal pathogenicity island. In this study, orthologue genes from several staphylococcal species, including , , , and , were identified, cloned and sequenced. Sequence analysis comparison of the gene from these species revealed a very high sequence similarity, suggesting the horizontal gene transfer of SaPIbov2 amongst them. However, sequence analyses of the flanking region revealed that the gene of these species was not contained in the SaPIbov2 pathogenicity island. Although they did not contain the operon, all the coagulase-negative staphylococcal isolates harbouring were strong biofilm producers. Disruption of the gene in abolished its capacity to form a biofilm, whereas heterologous complementation of a biofilm-negative strain of with the Bap protein from bestowed the capacity to form a biofilm on a polystyrene surface. Altogether, these results demonstrate that Bap orthologues from coagulase-negative staphylococci induce an alternative mechanism of biofilm formation that is independent of the PIA/PNAG exopolysaccharide.

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2005-07-01
2019-10-16
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