1887

Abstract

, a lactic acid bacterium widely used for food fermentations, is often exposed to damaging stress conditions. In particular, oxidative stress leads to DNA, protein and membrane damages that can be lethal. As has no catalase, the impact of production of the haem catalase KatE on its oxidative stress resistance was tested. This cytoplasmic catalase was engineered for extracellular expression in with an optimization strategy based on fusion to the nisin-inducible promoter and a lactococcal signal peptide (SP). The production of KatE by conferred an 800-fold increase in survival after 1 h exposure to 4 mM hydrogen peroxide, and a 160-fold greater survival in long-term (3 days) survival of aerated cultures in a mutant, which is unable to respire. The presence of KatE protected DNA from oxidative damage and limited its degradation after long-term aeration in a / mutant, defective in DNA repair. is thus able to produce active catalase that can provide efficient antioxidant activity.

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2005-09-01
2020-04-02
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References

  1. Abriouel H., Herrmann A., Starke J., Yousif N. M., Wijaya A., Tauscher B., Holzapfel W., Franz C. M. 2004; Cloning and heterologous expression of hematin-dependent catalase produced by Lactobacillus plantarum CNRZ 1228. Appl Environ Microbiol70:603–606[CrossRef]
    [Google Scholar]
  2. Berlett B. S., Stadtman E. R. 1997; Protein oxidation in aging, disease, and oxidative stress. J Biol Chem272:20313–20316[CrossRef]
    [Google Scholar]
  3. Bermudez-Humaran L. G., Langella P., Miyoshi A., Gruss A., Guerra R. T., Le Loir Y, Montes de Oca-Luna R.. 2002; Production of human papillomavirus type 16 E7 protein in Lactococcus lactis . Appl Environ Microbiol68:917–922[CrossRef]
    [Google Scholar]
  4. Bermudez-Humaran L. G., Langella P., Commissaire J., Gilbert S., Le Loir Y., L'Haridon R., Corthier G. 2003; Controlled intra- or extracellular production of staphylococcal nuclease and ovine omega interferon in Lactococcus lactis . FEMS Microbiol Lett224:307–313[CrossRef]
    [Google Scholar]
  5. Bruno-Barcena J. M., Andrus J. M., Libby S. L., Klaenhammer T. R., Hassan H. M. 2004; Expression of a heterologous manganese superoxide dismutase gene in intestinal lactobacilli provides protection against hydrogen peroxide toxicity. Appl Environ Microbiol70:4702–4710[CrossRef]
    [Google Scholar]
  6. Condon S. 1987; Responses of lactic acid bacteria to oxygen. FEMS Microbiology Lett46:269–280[CrossRef]
    [Google Scholar]
  7. de Ruyter P. G., Kuipers O. P., Beerthuyzen M. M., van Alen-Boerrigter I., de Vos W. M. 1996a; Functional analysis of promoters in the nisin gene cluster of Lactococcus lactis . J Bacteriol178:3434–3439
    [Google Scholar]
  8. de Ruyter P. G., Kuipers O. P., de Vos W. M. 1996b; Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl Environ Microbiol62:3662–3667
    [Google Scholar]
  9. Duwat P., Ehrlich S. D., Gruss A. 1995; The recA gene of Lactococcus lactis : characterization and involvement in oxidative and thermal stress. Mol Microbiol17:1121–1131[CrossRef]
    [Google Scholar]
  10. Duwat P., Sourice S., Cesselin B.. 7 other authors 2001; Respiration capacity of the fermenting bacterium Lactococcus lactis and its positive effects on growth and survival. J Bacteriol183:4509–4516[CrossRef]
    [Google Scholar]
  11. Engelmann S., Lindner C., Hecker M. 1995; Cloning, nucleotide sequence, and regulation of katE encoding a sigma B-dependent catalase in Bacillus subtilis . J Bacteriol177:5598–5605
    [Google Scholar]
  12. Farr S. B., Kogoma T. 1991; Oxidative stress responses in Escherichia coli and Salmonella typhimurium . Microbiol Rev55:561–585
    [Google Scholar]
  13. Fridovich I. 1998; Oxygen toxicity: a radical explanation. J Exp Biol201:1203–1209
    [Google Scholar]
  14. Gaudu P., Lamberet G., Poncet S., Gruss A. 2003; CcpA regulation of aerobic and respiration growth in Lactococcus lactis . Mol Microbiol50:183–192[CrossRef]
    [Google Scholar]
  15. Gasson M. J. 1983; Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J Bacteriol154:1–9
    [Google Scholar]
  16. Gibson T. J. 1984; Studies of the Eppstein – Barr virus genome PhD thesis University of Cambridge; UK:
    [Google Scholar]
  17. Igarashi T., Kono Y., Tanaka K. 1996; Molecular cloning of manganese catalase from Lactobacillus plantarum . J Biol Chem271:29521–29524[CrossRef]
    [Google Scholar]
  18. Imlay J. A. 2003; Pathways of oxidative damage. Annu Rev Microbiol57:395–418[CrossRef]
    [Google Scholar]
  19. Knauf H. J., Vogel R. F., Hammes W. P. 1992; Cloning, sequence, and phenotypic expression of katA , which encodes the catalase of Lactobacillus sake LTH677. Appl Environ Microbiol58:832–839
    [Google Scholar]
  20. Kruidenier L., Verspaget H. W. 2002; Review article: oxidative stress as a pathogenic factor in inflammatory bowel disease – radicals or ridiculous?. Aliment Pharmacol Ther16:1997–2015[CrossRef]
    [Google Scholar]
  21. Kruidenier L., Kuiper I., Lamers C. B., Verspaget H. W. 2003a; Intestinal oxidative damage in inflammatory bowel disease: semi-quantification, localization, and association with mucosal antioxidants. J Pathol201:28–36[CrossRef]
    [Google Scholar]
  22. Kruidenier L., Kuiper I., Marklund S. L., Lamers C. B., Verspaget H. W, van Duijn W., van Hogezand R. A.. 2003b; Differential mucosal expression of three superoxide dismutase isoforms in inflammatory bowel disease. J Pathol201:7–16[CrossRef]
    [Google Scholar]
  23. Kruidenier L., Kuiper I., Van Duijn W., Mieremet-Ooms M. A., Lamers C. B., Verspaget H. W, van Hogezand R. A.. 2003c; Imbalanced secondary mucosal antioxidant response in inflammatory bowel disease. J Pathol201:17–27[CrossRef]
    [Google Scholar]
  24. Kruidenier L., Kuiper I., Jaarsma D., Lamers C. B., Zijlstra F. J., Verspaget H. W, van Meeteren M. E.. 2003d; Attenuated mild colonic inflammation and improved survival from severe DSS-colitis of transgenic Cu/Zn-SOD mice. Free Radic Biol Med34:753–765[CrossRef]
    [Google Scholar]
  25. Kuipers O. P., Kleerezen M., Vos W. M. D, de Ruyters P. G.. 1998; Quorum sensing-controlled gene expression in lactic acid bacteria. J Biotechnol64:15–21[CrossRef]
    [Google Scholar]
  26. Kunst F., Ogasawara N., Moszer I.. 148 other authors 1997; The complete genome sequence of the gram-positive bacterium Bacillus subtilis . Nature390:249–256[CrossRef]
    [Google Scholar]
  27. Langella P., Le Loir Y., Ehrlich S. D., Gruss A. 1993; Efficient plasmid mobilization by pIP501 in Lactococcus lactis subsp. lactis . J Bacteriol175:5806–5813
    [Google Scholar]
  28. Le Loir Y., Gruss A., Ehrlich S. D., Langella P. 1994; Direct screening of recombinants in gram-positive bacteria using the secreted staphylococcal nuclease as a reporter. J Bacteriol176:5135–5139
    [Google Scholar]
  29. Le Loir Y., Gruss A., Ehrlich S. D., Langella P. 1998; A nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in Lactococcus lactis . J Bacteriol180:1895–1903
    [Google Scholar]
  30. Le Loir Y., Nouaille S., Commissaire J., Bretigny L., Gruss A., Langella P. 2001; Signal peptide and propeptide optimization for heterologous protein secretion in Lactococcus lactis . Appl Environ Microbiol67:4119–4127[CrossRef]
    [Google Scholar]
  31. Mares A., Neyts K., Debevere J. 1994; Influence of pH, salt and nitrite on the heme-dependent catalase activity of lactic acid bacteria. Int J Food Microbiol24:191–198[CrossRef]
    [Google Scholar]
  32. Noonpakdee W., Sitthimonchai S., Panyim S., Lertsiri S. 2004; Expression of the catalase gene katA in starter culture Lactobacillus plantarum TISTR850 tolerates oxidative stress and reduces lipid oxidation in fermented meat product. Int J Food Microbiol95:127–135[CrossRef]
    [Google Scholar]
  33. Nouaille S., Ribeiro L. A., Miyoshi A., Pontes D., Le Loir Y., Oliveira S. C., Langella P., Azevedo V. 2003; Heterologous protein production and delivery systems for Lactococcus lactis . Genet Mol Res2:102–111
    [Google Scholar]
  34. Pifferi P.-G. B.-V., Spagna G., Tramontini M. 1993; Immobilization of catalase on macromolecular supports activated with acid dyes. Process Biochem28:29–38[CrossRef]
    [Google Scholar]
  35. Renault P., Corthier G., Goupil N., Delorme C., Ehrlich S. D. 1996; Plasmid vectors for gram-positive bacteria switching from high to low copy number. Gene183:175–182[CrossRef]
    [Google Scholar]
  36. Rezaïki L., Cesselin B., Yamamoto Y., Vido K., Gaudu P., Gruss A, van West E.. 2004; Respiration metabolism reduces oxidative and acid stress to improve long-term survival of Lactococcus lactis . Mol Microbiol53:1331[CrossRef]
    [Google Scholar]
  37. Rochat T., Gratadoux J. J., Corthier G., Coqueran B., Nader-Macias M. E., Gruss A., Langella P. 2005; Lactococcus lactis SpOx spontaneous mutants: a family of oxidative-stress-resistant dairy strains. Appl Environ Microbiol71:2782–2788[CrossRef]
    [Google Scholar]
  38. Ross S. 1991; The involvement of oxygen radicals in microbicidal mechanism of leukocytes and macrophages. Klin Wochenschr69:975–980[CrossRef]
    [Google Scholar]
  39. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  40. Sanders J. W., Leenhouts K. J., Haandrikman A. J., Venema G., Kok J. 1995; Stress response in Lactococcus lactis : cloning, expression analysis, and mutation of the lactococcal superoxide dismutase gene. J Bacteriol177:5254–5260
    [Google Scholar]
  41. Simon D., Chopin A. 1988; Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis . Biochimie70:559–566[CrossRef]
    [Google Scholar]
  42. Simonen M., Palva I. 1993; Protein secretion in Bacillus species. Microbiol Rev57:109–137
    [Google Scholar]
  43. Sinha A. K. 1972; Colorimetric assay of catalase. Anal Biochem47:389–394[CrossRef]
    [Google Scholar]
  44. Storz G., Imlay J. A. 1999; Oxidative stress. Curr Opin Microbiol2:188–194[CrossRef]
    [Google Scholar]
  45. Sun J., Chen Y., Li M., Ge Z. 1998; Role of antioxidant enzymes on ionizing radiation resistance. Free Radic Biol Med24:586–593[CrossRef]
    [Google Scholar]
  46. van Asseldonk M., Rutten G., Oteman M., Siezen R. J., Simons G, de Vos W. M.. 1990; Cloning of usp45 , a gene encoding a secreted protein from Lactococcus lactis subsp lactis MG1363. Gene95155–160[CrossRef]
    [Google Scholar]
  47. van der Vossen J. M., van der Lelie D., Venema G. 1987; Isolation and characterization of Streptococcus cremoris Wg2-specific promoters. Appl Environ Microbiol53:2452–2457
    [Google Scholar]
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