1887

Abstract

Transposon mutagenesis of sp. PCC7120 led to the isolation of a mutant strain, PHB11, which grew poorly at pH values above 10. The mutant strain exhibited pronounced Na sensitivity; this sensitivity was higher under basic conditions. Mutant PHB11 also showed an inhibition of photosynthesis that was much more pronounced at alkaline pH. Reconstruction of the transposon mutation of PHB11 in the wild-type strain reproduced the phenotype of the original mutant. The wild-type version of the mutated gene was cloned and the mutation complemented. In mutant strain PHB11, the transposon had inserted within an ORF that is part of a seven-ORF operon with significant sequence similarity to a family of bacterial operons that are believed to code for a novel multiprotein cation/proton antiporter primarily involved in resistance to salt stress and adaptation to alkaline pH. The operon was denoted (multiple resistance and pH adaptation) following the nomenclature of the operon; the ORF mutated in PHB11 corresponded to . Computer analysis suggested that all seven predicted Mrp proteins were highly hydrophobic with several transmembrane domains; in fact, the predicted protein sequences encoded by , and showed significant similarity to hydrophobic subunits of the proton pumping NADH : ubiquinone oxidoreductase. expression studies indicated that is induced with increasing external Na concentrations and alkaline pH; is also upregulated under inorganic carbon (Ci) limitation. The biological significance of a putative cyanobacterial Mrp complex is discussed.

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2005-05-01
2019-10-21
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Several characteristics of the putative gene products of the locus and their predicted similarity to known protein sequences [PDF file](25 KB)

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Alignment of MrpA from sp. strain PCC7120 and selected bacterial species [PDF file](48 KB)

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Schematic diagram of the region of sp. strain PCC7120 showing the ORF denominations [PDF file](110 KB)

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Sequence alignment of the presumptive LysR-type T(N11)A motif binding sites in the promoter region of the putative all1843–all1837 operon [PDF file](12 KB)

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