1887

Abstract

The aim of this study was to evaluate the use of RNA polymerase subunit () and phenylalanyl-tRNA synthase () gene sequences as species identification tools for enterococci. Ninety-six representative strains comprising all currently recognized species were examined. gene sequences generated a robust classification into species groups similar to the one based on 16S rRNA gene sequence analysis. On the other hand, the gene is a fast-evolving clock even better suited for species delineation than the gene, but not for recognition of species groups within as determined by both and 16S rRNA genes. All enterococcal species were clearly differentiated on the basis of their and sequences. Evaluation of intraspecies variation showed that both and genes have a high degree of homogeneity among strains of the same species. Strains of the same enterococcal species have at least 99 % and 97 % gene sequence similarity, whereas, different enterococcal species have at maximum 97 % and 86 % gene sequence similarity. It was concluded that both genes can be used as reliable tools for identification of clinical and environmental species of and are efficient screening methods for the detection of novel species. The sequence data obtained in this study were compared to the available and 16S rRNA gene sequences. The MLSA approach to taxonomy provides portable, highly reproducible data with lower costs for rapid identification of all enterococcal species.

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2005-07-01
2020-01-26
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