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Volume 151, Issue 6

Up-regulation of competence- but not stress-responsive proteins accompanies an altered metabolic phenotype in biofilms Free

Abstract

Mature biofilm and planktonic cells of cultured in a neutral pH environment were subjected to comparative proteome analysis. Of the 242 protein spots identified, 48 were significantly altered in their level of expression (<0·050) or were unique to planktonic or biofilm-grown cells. Among these were four hypothetical proteins as well as proteins known to be associated with the maintenance of competence or found to possess a -box-like element upstream of their coding gene. Most notable among the non-responsive genes were those encoding the molecular chaperones DnaK, GroEL and GroES, which are considered to be up-regulated by sessile growth. Analysis of the rest of the proteome indicated that a number of cellular functions associated with carbon uptake and cell division were down-regulated. The data obtained were consistent with the hypothesis that a reduction in the general growth rate of mature biofilms of in a neutral pH environment is associated with the maintenance of transformation without the concomitant stress response observed during the transient state of competence in bacterial batch cultures.

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  • Accepted:
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  • Published Online:
Keyword(s): 2-DGE, 2-dimensional gel electrophoresis , 2D, 2-dimensional , CcpA, catabolite control protein A , CSP, competence-stimulating peptide , D, dilution rate , IPG, immobilized pH gradient , MALDI, matrix-assisted laser desorption ionization , PMM, peptide mass mapping and TOF, time-of-flight
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2005-06-01
2023-03-21
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Up-regulation of competence- but not stress-responsive proteins accompanies an altered metabolic phenotype in Streptococcus mutans biofilms
Microbiology 151, 1823 (2005); https://doi.org/10.1099/mic.0.27830-0
/content/journal/micro/10.1099/mic.0.27830-0
/content/journal/micro/10.1099/mic.0.27830-0
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