1887

Abstract

The essential mechanisms and virulence factors enabling species to survive and replicate inside host macrophages are not fully understood. The authors previously reported that a putative guanosine 5′-diphosphate 3′-diphosphate (ppGpp) mutant ( mutant) of failed to replicate in HeLa cells. The present study showed that the pattern of surface proteins and morphological change of the mutant were different from wild-type. wild-type changed its morphology upon treatment with ppGpp synthetase I activation inhibitor. In various tests under stress conditions, including nutrient starvation, nitric oxide resistance, acid resistance and antibiotic resistance, the mutant had a lower stress resistance than wild-type. Although the mutant has the same smooth phenotype and LPS profile as wild-type, it had a higher rate of adherence to macrophages but lower internalization and intracellular replication within macrophages. The mutant did not co-localize with either late endosomes or lysosomes and was almost cleared from the spleens of mice after 10 days, without splenomegaly. RT-PCR was used to detect mRNA from around 10 cells incubated in low-pH enriched medium; it showed that the expression of increased after 30 min incubation. The data suggest that SpoT does not contribute to intracellular trafficking of , but contributes to the maintenance of bacterial morphology and the physiological adaptation required for intracellular replication.

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2005-05-01
2020-04-06
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