1887

Abstract

This study describes the cloning, genetic analysis and biochemical characterization of a leucyl aminopeptidase (LAP) from . A gene encoding LAP was cloned from and the expressed 55 kDa protein displayed homology to aminopeptidases from Gram-negative bacteria, plants and mammals. This LAP demonstrated amidolytic activity against -leucine--nitroanilide. Optimal activity was observed at pH 8·0 and 45 °C, with of 232 μmol min (mg protein) and of 0·65 mM. The data suggest that LAP could be allosteric ( =2·27), with regulatory homohexamers, and its activity was inhibited by ion chelators and enhanced by divalent manganese, cobalt and nickel cations. Bestatin inhibited both LAP activity (IC=49·9 nM) and growth . The results point to the potential use of LAP as a drug target to develop novel anti- agents.

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2005-06-01
2020-03-29
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