1887

Abstract

CMUAc130 was isolated from the root tissue of Rosc. (Zingiberaceae). It was an antagonist of and , the causative agents of anthracnose of banana and wilt of wheat, respectively. Evidence for the antibiosis of CMUAc130 was demonstrated by the zone of fungal-growth inhibition. Microscopic observations showed thickness and bulbous structures at the edges of the inhibited fungal hyphae. The culture filtrate and crude extract from this strain were all inhibitory to tested phytopathogenic fungi. The major active ingredients from the culture filtrate of CMUAc130 were purified by silica gel-column chromatography and identified to be (i) 5,7-dimethoxy-4--methoxylphenylcoumarin and (ii) 5,7-dimethoxy-4-phenylcoumarin by NMR and mass-spectral data, respectively. Bioassay studies showed that compounds (i) and (ii) had antifungal activities against tested fungi, and their MICs were found to be 120 and 150 μg ml, respectively. This is the first report of compounds (i) and (ii) from micro-organisms as active ingredients for the control of phytopathogenic fungi.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.27758-0
2005-05-01
2020-04-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/151/5/mic1511691.html?itemId=/content/journal/micro/10.1099/mic.0.27758-0&mimeType=html&fmt=ahah

References

  1. Delle Monache G., Botta B., Neto A. S., Lima R. A. 1983; 4-Arylcoumarins from Coutarea hexandra. Phytochemistry22:1657–1658[CrossRef]
    [Google Scholar]
  2. El-Shanshoury A. E. R., El-Sououd S. M. A., Awadalla O. A., El-Bandy N. B. 1996; Effects of Streptomyces corchorusii, Streptomyces mutabilis, pendimethalin, and metribuzin on the control of bacterial and Fusarium wilt of tomato. Can J Bot74:1016–1022[CrossRef]
    [Google Scholar]
  3. Elson M. K., Kelly J. F., Nair M. G. 1994; Influence of antifungal compounds from a soil-borne actinomycete on Fusarium spp. in asparagus. J Chem Ecol20:2835–2846[CrossRef]
    [Google Scholar]
  4. Katznelson H., Cole S. E. 1965; Production of gibberellin-like substances by bacteria and actinomycetes. Can J Microbiol11:733–741[CrossRef]
    [Google Scholar]
  5. Mata R., Calzada F., Garcia M. R. 1988; Chemical studies on Mexican plants used in traditional medicine. VI. Additional new 4-phenylcoumarins from Exostema caribaeum. J Nat Prod51:851–856[CrossRef]
    [Google Scholar]
  6. Mata R., del Rayo Camacho M., Cervera E., Bye R., Linares E. 1990; Secondary metabolites from Hintonia latiflora. Phytochemistry29:2037–2040[CrossRef]
    [Google Scholar]
  7. Mishra S. K., Taft W. H., Putnam A. R., Ries S. K. 1987; Plant growth regulatory metabolites from novel actinomycetes. J Plant Growth Regul6:75–83[CrossRef]
    [Google Scholar]
  8. Rothrock C. S., Gottlieb D. 1984; Role of antibiosis in antagonism of Streptomyces hygroscopicus var.geldanus to Rhizoctonia solani in soil. Can J Microbiol30:1440–1447[CrossRef]
    [Google Scholar]
  9. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol16:313–340[CrossRef]
    [Google Scholar]
  10. Taechowisan T., Lumyong S. 2003; Activity of endophytic actinomycetes from roots of Zingiber officinale and Alpinia galanga against phytopathogenic fungi. Ann Microbiol53:291–298
    [Google Scholar]
  11. Taechowisan T., Peberdy J. F., Lumyong S. 2003; Isolation of endophytic actinomycetes from selected plants and their antifungal activity. World J Microbiol Biotechnol19:381–385[CrossRef]
    [Google Scholar]
  12. Tahvonen R. 1982a; Preliminary experiments in the use of Streptomyces spp. isolated from peat in the biological control of soil and seed-borne diseases in peat culture. J Agric Sci Finl54:357–369
    [Google Scholar]
  13. Tahvonen R. 1982b; The suppressiveness of Finnish light coloured Sphagnum peat. J Agric Sci Finl54:345–356
    [Google Scholar]
  14. Tahvonen R. 1993; The disease suppressiveness of light-coloured sphagnum peat and biocontrol of plant diseases with Streptomyces sp. Acta Hortic342:37–42
    [Google Scholar]
  15. Trejo-Estrada S. R., Sepulveda I. R., Crawford D. L. 1998; In vitro and in vivo antagonism of Streptomyces violaceusniger YCED9 against fungal pathogens of turfgrass. World J Microbiol Biotechnol14:865–872[CrossRef]
    [Google Scholar]
  16. Tuomi T., Heino M., Nordstrom K., Laakso S. 2001; Fiber fractions from processing of barley in production and conservation of a biologic control agent. Appl Biochem Biotechnol94:135–145[CrossRef]
    [Google Scholar]
  17. Yuan W. M., Crawford D. L. 1995; Characterization of Streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. Appl Environ Microbiol61:3119–3128
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.27758-0
Loading
/content/journal/micro/10.1099/mic.0.27758-0
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error