1887

Abstract

CMUAc130 was isolated from the root tissue of Rosc. (Zingiberaceae). It was an antagonist of and , the causative agents of anthracnose of banana and wilt of wheat, respectively. Evidence for the antibiosis of CMUAc130 was demonstrated by the zone of fungal-growth inhibition. Microscopic observations showed thickness and bulbous structures at the edges of the inhibited fungal hyphae. The culture filtrate and crude extract from this strain were all inhibitory to tested phytopathogenic fungi. The major active ingredients from the culture filtrate of CMUAc130 were purified by silica gel-column chromatography and identified to be (i) 5,7-dimethoxy-4--methoxylphenylcoumarin and (ii) 5,7-dimethoxy-4-phenylcoumarin by NMR and mass-spectral data, respectively. Bioassay studies showed that compounds (i) and (ii) had antifungal activities against tested fungi, and their MICs were found to be 120 and 150 μg ml, respectively. This is the first report of compounds (i) and (ii) from micro-organisms as active ingredients for the control of phytopathogenic fungi.

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2005-05-01
2019-10-14
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