%0 Journal Article %A Gaines, Jennifer M. %A Carty, Nancy L. %A Colmer-Hamood, Jane A. %A Hamood, Abdul N. %T Effect of static growth and different levels of environmental oxygen on toxA and ptxR expression in the Pseudomonas aeruginosa strain PAO1 %D 2005 %J Microbiology, %V 151 %N 7 %P 2263-2275 %@ 1465-2080 %R https://doi.org/10.1099/mic.0.27754-0 %K ETA, exotoxin A %K CF, cystic fibrosis %K 20 %-ST, aerobic and static %K 0 %-ST, anaerobic (0 % EO) and static %K 20 %-SH, aerobic (20 % EO) with shaking %K 10 %-ST, microaerobic (10 % EO) and static %K DO, dissolved oxygen %K Fur, ferric uptake regulator %K EO, environmental oxygen %I Microbiology Society, %X Within certain infection sites, such as the lung of cystic fibrosis patients, Pseudomonas aeruginosa grows statically under either decreased oxygen tension or anaerobic conditions, a situation that is likely to influence the production of virulence factors. The goal of this study was to determine the effect of static growth under microaerobic (decreased oxygen) and anaerobic conditions on the expression of the P. aeruginosa exotoxin A (ETA) gene toxA and its positive regulator ptxR. Using toxA–lacZ and ptxR–lacZ fusion plasmids, the level of toxA and ptxR expression was measured throughout the growth cycle of strain PAO1, which was grown in either iron-deficient or iron-sufficient medium under four different conditions: 20 %-SH (aerobic, shaking), 20 %-ST (aerobic, static), 10 %-ST (microaerobic, static) and 0 %-ST (anaerobic, static). In iron-deficient medium, toxA expression was higher under 20 %-ST and 10 %-ST than under 20 %-SH. However, the highest level of toxA expression occurred under 0 %-ST. Analysis of ETA protein using sandwich ELISA revealed that at time points between 8 and 24 h of the growth curve, PAO1 produced higher levels of ETA under 0 %-ST than under 20 %-SH. In iron-sufficient medium, toxA expression was significantly repressed under all conditions. Additional analyses using PAO1 strains that carry lacZ fusions with the toxA regulatory genes regA and pvdS revealed that the expression of regA and pvdS is reduced rather than increased at 0 %-ST. ptxR expression under different conditions paralleled that of toxA expression, except that it was repressed by iron under 20 %-SH only. Between 6 and 24 h of growth, and under all conditions, the level of dissolved oxygen (DO) within the PAO1 cultures was sharply reduced. These results suggest that (1) the combined effect of static growth and anaerobic conditions produce a significant increase in toxA and ptxR expression in PAO1; (2) this effect appears to be unique to toxA and ptxR, since the level of regA and pvdS expression was reduced under the same conditions; (3) neither static growth nor anaerobic conditions interfere with the repression of toxA expression by iron, although static growth deregulates ptxR expression with respect to iron; and (4) the enhanced expression of toxA and ptxR is not related to the reduced levels of DO in PAO1 cultures. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.27754-0