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Volume 151, Issue 5

Allosteric NADP-glutamate dehydrogenase from aspergilli: purification, characterization and implications for metabolic regulation at the carbon–nitrogen interface Free

Abstract

NADP-dependent glutamate dehydrogenase (NADP-GDH) mediates fungal ammonium assimilation through reductive synthesis of glutamate from 2-oxoglutarate. By virtue of its position at the interface of carbon and nitrogen metabolism, biosynthetic NADP-GDH is a potential candidate for metabolic control. In order to facilitate characterization, a new and effective dye-affinity method was devised to purify NADP-GDH from two aspergilli, and . The NADP-GDH was characterized at length and its kinetic interaction constants with glutamate ( 34·7 mM) and ammonium ( 1·05 mM; 0·4 mM) were consistent with an anabolic role. Isophthalate, 2-methyleneglutarate and 2,4-pyridinedicarboxylate were significant inhibitors, with respective values of 6·9, 9·2 and 202·0 μM. The enzyme showed allosteric properties and a sigmoid response ( =2·5) towards 2-oxoglutarate saturation. The co-operative behaviour was a feature common to NADP-GDH from , and . NADP-GDH may therefore be a crucial determinant in adjusting 2-oxoglutarate flux between the tricarboxylic acid cycle and glutamate biosynthesis in aspergilli.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): GDH, glutamate dehydrogenase and TCA, tricarboxylic acid
SGM
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2005-05-01
2024-04-20
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Allosteric NADP-glutamate dehydrogenase from aspergilli: purification, characterization and implications for metabolic regulation at the carbon–nitrogen interface
Microbiology 151, 1409 (2005); https://doi.org/10.1099/mic.0.27751-0
/content/journal/micro/10.1099/mic.0.27751-0
/content/journal/micro/10.1099/mic.0.27751-0
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