1887

Abstract

-Glucuronidase activity (encoded by the gene) has been characterized for the first time from E1, an anaerobic bacterium belonging to the dominant human gut microbiota. -Glucuronidase activity plays a major role in the generation of toxic and carcinogenic metabolites in the large intestine, as well as in the absorption and enterohepatic circulation of many aglycone residues with protective effects, such as lignans, flavonoids, ceramide and glycyrrhetinic acid, that are liberated by the hydrolysis of the corresponding glucuronides. The complete nucleotide sequence of a 4537 bp DNA fragment containing the -glucuronidase locus from E1 was determined. Five ORFs were detected on this fragment: three complete ORFs (ORF2, and ORF3) and two partial ORFs (ORF4 and ORF5). The products of ORF2 and ORF3 show strong similarities with many -glucoside permeases of the phosphoenolpyruvate : -glucoside phosphotransferase systems (PTSs), such as BglC, BglP and PTS Enzyme II. The product of ORF5 presents strong similarities with the amino-terminal domain of -glucosidase (). The gene product presents similarities with several known -glucuronidase enzymes, including those of (69 %), (61 %), (59 %) and (58 %). By complementing an strain in which the gene encoding the enzyme was deleted, it was confirmed that the gene encodes the -glucuronidase enzyme. Moreover, it was found that the gene was transcribed as part of an operon that includes ORF2, ORF3 and ORF5.

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2005-07-01
2020-04-08
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