1887

Abstract

The opportunistic bacterium C5424 contains two catalase/peroxidase genes, and . To investigate the functions of these genes, and mutants were generated by targeted integration of suicide plasmids into the and genes. The catalase/peroxidase activity of the mutant was not affected as compared with that of the parental strain, while no catalase/peroxidase activity was detected in the mutant. However, the mutant displayed reduced resistance to hydrogen peroxide under iron limitation, while the mutant showed hypersensitivity to hydrogen peroxide, and reduced growth under all conditions tested. The mutant displayed reduced growth only in the presence of carbon sources that are metabolized through the tricarboxylic acid (TCA) cycle, as the growth defect was abrogated in cultures supplemented with glucose or glycerol. This phenotype was also correlated with a marked reduction in aconitase activity. In contrast, aconitase activity was not reduced in the mutant and parental strains. The authors conclude that the KatA protein is a specialized catalase/peroxidase that has a novel function by contributing to maintain the normal activity of the TCA cycle, while KatB is a classical catalase/peroxidase that plays a global role in cellular protection against oxidative stress.

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2005-06-01
2019-10-15
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