The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 μM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of these two regulons increases significantly. Other regulons with increased transcription in response to elevated concentrations of copper-glycine include those for the superoxide stress response, iron homeostasis, and envelope stress. Furthermore, a variety of ORFs with decreased expression in response to increased copper-glycine has been identified, including the zinc ABC transporter and genes involved in the chemotactic response.
BarthM.,
MarschallC.,
MufflerA.,
FischerD.,
Hengge-AronisR.
1995; Role for the histone-like protein H-NS in growth phase-dependent and osmotic regulation of σs and many σs-dependent genes in Escherichia coli. J Bacteriol 177:3455–3464
BenjaminiY.,
HochbergY.
1995; Controlling the false discovery rate – a practical and powerful approach to multiple testing. J R Stat Soc Ser B Methodol 57:289–300
BertinP.,
TeraoE.,
LeeE. H.,
LejeuneP.,
ColsonC.,
DanchinA.,
CollatzE.
1994; The H-NS protein is involved in the biogenesis of flagella in Escherichia coli
. J Bacteriol 176:5537–5540
BrickmanT. J.,
OzenbergerB. A.,
McIntoshM. A.
1990; Regulation of divergent transcription from the iron-responsive fepB-entC promoter-operator regions in Escherichia coli. J Mol Biol 212:669–682[CrossRef]
BrownN. L.,
LeeB. T. O.,
SilverS.
1994; Bacterial transport of and resistance to copper. In Metal Ions in Biological Systems pp 405–434 Edited by
SigelH.,
SigelA.
New York: Marcel Dekker;
BrowningD. F.,
ColeJ. A.,
BusbyS. J. W.
2000; Supression of FNR-dependent transcription activation at the Escherichia coli nir promoter by Fis, IHF and H-NS: modulation of transcription initiation by a complex nucleo-protein assembly. Mol Microbiol 37:1258–1269[CrossRef]
DaneseP. N.,
SnyderW. B.,
CosmaC. L.,
DavisL. J.,
SilhavyT. J.
1995; The Cpx two-component signal transduction pathway of Escherichia coli regulates transcription of the gene specifying the stress-inducible periplasmic protease. Genes Dev 9:387–398[CrossRef]
DartigalongueC.,
RainaS.
1998; A new heat-shock gene, ppiD, encodes a peptidyl-prolyl isomerase required for folding of outer membrane proteins in Escherichia coli. EMBO J 17:3968–3980[CrossRef]
DatsenkoK. A.,
WannerB. L.
2000; One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97:6640–6645[CrossRef]
De WulfP.,
KwonO.,
LinE. C. C.
1999; The CpxRA signal transduction system of Escherichia coli: growth related autoactivation and control of unanticipated target operons. J Bacteriol 181:6772–6778
De WulfP.,
McGuireA. M.,
LiuX.,
LinE. C. C.
2002; Genome-wide profiling of promoter recognition by the two-component response regulator CpxR-P in Escherichia coli. J Biol Chem 277:26652–26661[CrossRef]
DorelC.,
VidalO.,
Prigent-CombaretC.,
ValletI.,
LejeuneP.
1999; Involvement of the Cpx signal transduction pathway of E. coli in biofilm formation. FEMS Microbiol Lett 178:169–175[CrossRef]
FawcettW. P.,
WolfR. E., Jr.
1995; Genetic definition of the Escherichia coli zwf “Soxbox”, the DNA binding site for SoxS-mediated induction of glucose 6-phosphate dehydrogenase in response to superoxide. J Bacteriol 177:1742–1750
FrankeS.,
GrassG.,
RensingC.,
NiesD. H.
2003; Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli. J Bacteriol 185:3804–3812[CrossRef]
FreeA.,
DormanC. J.
1997; The Escherichia coli stpA gene is transiently expressed during growth in rich medium and is induced in minimal medium and by stress conditions. J Bacteriol 179:909–918
GovantesF.,
OrjaloA. V.,
GunsalusR. P.
2000; Interplay between three global regulatory proteins mediates oxygen regulation of the Escherichia coli cytochrome doxidase (cydAB) operon. Mol Microbiol 38:1061–1073
GrassG.,
ThakaliK.,
KlebbaP. E.,
ThiemeD.,
MullerA.,
WildnerG. F.,
RensingC.
2004; Linkage between catecholate siderophores and the multicopper oxidase CueO in Escherichia coli. J Bacteriol 186:5826–5833[CrossRef]
GrossC.,
KelleherM.,
IyerV. R.,
BrownP. O.,
WingeD. R.
2000; Identification of the copper regulon in Saccharomyces cerevisiae by DNA microarrays. J Biol Chem 275:32310–32316[CrossRef]
HarrisonM. D.,
JonesC. E.,
SoliozM.,
DameronC. T.
2000; Intracellular copper routing: the role of copper chaperones. Trends Biochem Sci 25:29–32[CrossRef]
LiuX.,
MatsumuraP.
1994; The FlhD/FlhC complex, a transcriptional activator of the Escherichia coli flagellar class II operons. J Bacteriol 176:7345–7351
LuchtJ. M.,
DerschP.,
KempfB.,
BremerE.
1994; Interactions of the nucleoid-associated DNA-binding protein H-NS with the regulatory region of the osmotically controlled proU operon of Escherichia coli. J Biol Chem 269:6578–6586
McHughJ. P.,
Rodriguez-QuinonesF.,
Abdul-TehraniH.,
SvistunenkoD. A.,
PooleR. K.,
CooperC. E.,
AndrewsS. C.
2003; Global iron-dependent gene regulation in Escherichia coli – a new mechanism for iron homeostasis. J Biol Chem 278:29478–29486[CrossRef]
MunsonG. P.,
LamD. L.,
OuttenF. W.,
O'HalloranT. V.
2000; Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12. J Bacteriol 182:5864–5871[CrossRef]
OlsenP. B.,
KlemmP.
1994; Localization of promoters in the fim gene cluster and the efffect of H-NS on the transcription offimB and fimE. FEMS Microbiol Lett 116:95–100[CrossRef]
OuttenF. W.,
OuttenC. E.,
HaleJ.,
O'HalloranT.
2000; Transcriptional activation of an Escherichia coli copper efflux regulon by the chromosomal MerR homologue, CueR. J Biol Chem 275:31024–31029[CrossRef]
OuttenF. W.,
HuffmanD. L.,
HaleJ. A.,
O'HalloranT. V.
2001; The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth inEscherichia coli. J Biol Chem 276:30670–30677[CrossRef]
PatzerS. I.,
HantkeK.
1998; The ZnuABC high-affinity zinc uptake system and its regulator Zur in Escherichia coli. Mol Microbiol 28:1199–1210[CrossRef]
PatzerS. I.,
HantkeK.
2000; The Zinc-responsive regulator Zur and its control of the znu gene cluster encoding the ZnuABC zinc uptake system inEscherichia coli. J Biol Chem 275:24321–24332[CrossRef]
PetersonC.,
MollerL. B.
2000; Control of copper homeostsis in Escherichia coli by a P-type ATPase, CopA, and a MerR-like transcriptional activator, CopR. Gene 261:289–298[CrossRef]
RobertsS. A.,
WeichselA.,
GrassG.,
ThakaliK.,
HazzardJ. T.,
TollinG.,
RensingC.,
MontfortW. R.
2002; Crystal structure and electron transfer kenetics of CueO, a multicopper oxidase required for copper homeostasis in Escherichia coli. Proc Natl Acad Sci U S A 99:2766–2771[CrossRef]
RoeschP. L.,
BlomfieldI. C.
1998; Leucine alters the interaction of the leucine-responsive regulatory protein (Lrp) with the fim switch to stimulate site-specific recombination in Escherichia coli. Mol Microbiol 27:751–761[CrossRef]
SchembriM. A.,
OlsenP. B.,
KlemmP.
1998; Orientation-dependent enhancement by H-NS of the activity of the type 1 fimbrial phase switch promoter in Escherichia coli. Mol Gen Genet 259:336–344[CrossRef]
ShimohataN.,
ChibaS.,
SaikawaN.,
ItoK.,
AkiyamaY.
2002; The Cpx stress response system of Escherichia coli senses plasma membrane proteins and controls HtpX, a membrane protease with a cytosolic active site. Genes Cells 7:653–662[CrossRef]
StoyanovJ. V.,
HobmanJ. L.,
BrownN. L.
2001; CueR (YbbI) of Escherichia coli is a MerR family regulator controlling expression of the copper exporter CopA. Mol Microbiol 39:502–511[CrossRef]
TramontiA.,
ViscaP.,
De CanioM.,
FalconiM.,
De BiaseD.
2002; Functional characterization and regulation of gadX, a gene encoding an AraC/XylS-like transcriptional activator of the Escherichia coli glutamic acid decarboxylase system. J Bacteriol 184:2603–2613[CrossRef]
ZhangL.,
ChaudhuriR. R.,
ConstantinidouC.12 other authors2004; Regulators encoded in the ETT2 gene cluster influence expression of genes within the locus of enterocyte effacement in enterohemorrhagic O157 : H7. Infect Immun 72:7282–7293[CrossRef]
ZhengD.,
ConstantinidouC.,
HobmanJ. L.,
MinchinS. D.
2004; Identification of the CRP regulon using in vitro and in vivo transcriptional profiling. Nucleic Acids Res 32:5874–5893[CrossRef]