1887

Abstract

encodes the major catalase that accounts for 90 % of the total catalase activity present in pv. . is located upstream of an ORF designated encoding a cytoplasmic membrane protein homologous to eukaryotic ankyrin. Transcriptional analysis of and identified two transcripts: a major monocistronic transcript and a minor bicistronic transcript. KatA expression was induced in the presence of various oxidants including HO, organic hydroperoxides and the superoxide-generating agent menadione, in an OxyR-dependent manner. Analysis of the promoter region showed a putative OxyR binding site located upstream of an -like −35 region that is likely to be responsible for transcription activation in response to oxidant treatment. Gel mobility shift experiments confirmed that purified OxyR specifically binds to the promoter. A mutant was highly sensitive to HO during both the exponential and stationary phases of growth. This phenotype could be complemented by functional , confirming the essential role of the gene in protecting from HO toxicity. Unexpectedly, inactivation of also significantly reduced resistance to HO and the phenotype could be complemented by plasmid-borne expression of . Physiological analyses showed that plays an important role in, but is not solely responsible for, both the adaptive and menadione-induced cross-protective responses to HO killing in .

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2005-02-01
2019-10-23
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