1887

Abstract

A new insertion sequence from ATCC 14999 was isolated and characterized. This IS element, designated IS, comprised a 1149 bp nucleotide sequence with 22 bp imperfect terminal inverted repeats. IS carries a single open reading frame of 345 amino acids encoding a putative transposase that appears to have partial homology to IS, an IS/Tc superfamily element, at the C-terminal region in the amino acid sequence. This indicated that IS belonged to the IS/Tc superfamily, which was first identified in . IS has a unique distance of 38 amino acid residues between the second and third amino acids in the DDE motif, which is well known as the catalytic centre of transposase. This suggested that IS constituted a new subfamily of the IS/Tc superfamily. A phylogenetic tree constructed on the basis of amino acid sequences of transposases revealed that this new transposable element was more similar to eukaryotic Tc/ family elements than to prokaryotic IS family elements. Added to the fact that IS was present in only a few strains, this implies that IS was probably acquired by a recent lateral transfer event from eukaryotic cells. Analysis of the insertion site in R revealed that IS appeared to transpose at random and always caused a target duplication of a 5′-TA-3′ dinucleotide upon insertion, like the other IS/Tc family elements. These findings indicated that IS could be a powerful tool for genetic manipulation of corynebacteria and related species.

Keyword(s): IR, inverted repeat
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2005-02-01
2019-11-16
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