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Volume 151, Issue 4

The hydrogenases of : a comparative genomic perspective Free

Abstract

The hydrogenase content of the genome of , a member of the family within the -subdivision of the , was examined and found to be distinct from that of species, another family of - on which extensive research concerning hydrogen metabolism has been conducted. Four [NiFe]-hydrogenases are encoded in the genome: two periplasmically oriented, membrane-bound hydrogenases, Hya and Hyb, and two cytoplasmic hydrogenases, Mvh and Hox. None of these [NiFe]-hydrogenases has a counterpart in species. Furthermore, the large and small subunits of Mvh and Hox appear to be related to archaeal and cyanobacterial hydrogenases, respectively. Clusters encoding [Fe]-hydrogenases and periplasmic [NiFeSe]-hydrogenases, which are commonly found in the genomes of species, are not present in the genome of . Hydrogen-evolving Ech hydrogenases, which are present in the genomes of at least two species, were also absent from the genome, despite the fact that is capable of hydrogen production. Instead, the genome contained a cluster encoding a multimeric ch ydrogenase elated (Ehr) complex that was similar in content to operons encoding Ech hydrogenases, but did not appear to encode a hydrogenase. Phylogenetic analysis revealed that the cluster is part of a family of related clusters found in both the Archaea and Bacteria.

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2005-04-01
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The hydrogenases of Geobacter sulfurreducens: a comparative genomic perspective
Microbiology 151, 1239 (2005); https://doi.org/10.1099/mic.0.27535-0
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